鱼腥草素钠对人脐静脉内皮细胞NO合成及NOS活力的影响

Protected effects of Sodium Houttuyfonate against HUVECs injury induced by LPS relying on NO-NOS signal pathway in vitro

目的:研究鱼腥草素钠对脂多糖(LPS)诱导的人脐静脉内皮细胞(HUVECs)NO合成及一氧化氮合酶(NOS)活力的影响.方法:采用15μg/ml LPS作用于HUVECs,共同孵育12h建立炎性损伤细胞模型.考察不同剂量鱼腥草素钠对各组HUVECs细胞存活率、培养上清液中LDH活力、NO含量、内皮型NOS(eNOS)及诱导型NOS(iNOS)表达的影响.结果:鱼腥草素钠可明显提高HUVECs细胞存活率,增加NO的分泌与释放,提高eNOS与降低iNOS活力,从而保护脂多糖诱导的HUVECs炎性损伤.结论:鱼腥草素钠对脂多糖诱导损伤的内皮细胞具有保护作用,其机制与其促进内皮细胞NO分泌与释放,增强eNOS活性和表达,改善血管内皮功能状态相关.

Objective： To investigate the protected effects of Sodium Houttuyfonate against Human Umbilical Vein Endothelial Cells（HUVECs） injury induced by Lipopolysaccharide（LPS）, and further explore the mechanism involved in NO-NOS signal pathway. Methods： The HUVECs injury model was reproduced by exposure with LPS（15 μg/ml, 12h）. The cell viability and toxicity was assayed with MTT and LDH releasing. The NO contents were determined by biochemical enzymological methods, and eNOS and iNOS were measured by Elisa. Results： The LPS with HUVECs, sodium houttuyfonate can obviously enhance cell survival rate, NO and eNOS contents in supernatant, decrease the activity of LDH and iNOS activity. Conclusion： Sodium houttuyfonate protects LPS-induced endothelial dysfunction, and the mechanism may involve modulating NO-NOS pathway.