盆炎消灌肠液改善小鼠微循环障碍的实验研究

Experimental Study of Pen Yan Xiao Enema Improving Microcirculation in Mice

目的:探讨盆炎消灌肠液对小鼠微循环障碍的改善作用。方法:选用小鼠60只,随机分为对照组、模型组、盆炎消灌肠液(2.5,5.0,10.0 g/kg)3个剂量组和盐酸川芎嗪组共6组,每组10只。采用高分子右旋糖酐建立小鼠微循环障碍模型。应用WX-9型微循环显微镜和微循环检测分析系统,观察小鼠微循环变化,观察在给药前及给药后5,10,20,30 min不同时间点,毛细血管管径大小、血液流速、血液流态及红细胞聚集性等指标的变化。结果:盆炎消灌肠液3个剂量组在给药后不同时间点均能改善微循环障碍,增大毛细血管管径,加快血液流速,改善血液流态,降低红细胞聚集性。随着剂量增加和给药时间的延长,改善微循环障碍的作用更加明显,效果更好。大剂量(10.0 g/kg)组与模型组比较差异有统计学意义(P<0.05)。结论:盆炎消灌肠液能明显对抗由高分子右旋糖酐所致小鼠微循环障碍,改善盆腔微循环。

Objective： To explore the improved effect of the Pen YaM Xiao enema on microcirculation in mice. Methods：Sixty adult mice were used. Ten mice were used as control group. Fifty mice were induced with the high-molecular dextran to develop animal models of microcirculation disturbance, and then randomly divided into 5 groups as follows, model group, three groups treated with Pen YaM Xiao enema liquid（2.5, 5.0, 10.0 g/kg）, and a group treated with Chuanxiongzine as positive control. Microcirculation in mice was compared before treat- ment and 5, 10, 20, 30 min after treatment using the WX-9 microcirculation microscope and the microcirculation detection and analysis system. Those parameters were analyzed, including the diameter size of capillary, the blood flow velocity, the blood flow pattern and the red cell aggregation. Results：Pen YaM Xiao enema can im- prove microcirculation in the model mice in a dose-dependent manner and a time-dependent manner. In three treatment groups, the diameter size of capillary and the blood flow velocity were increased; the blood flow pattern was improved, while the red cell aggregation was decreased. Those effects of large doses （10.0 g/kg） of Pen YaM Xiao enema were significant （P〈0.05）. Conclusions ：Pen YaM Xiao enema can significantly improve microcircu- lation in the animal model of microcirculation disturbance induced by high polymer dextran, suggesting the phar- macologic action of Pen YaM Xiao on microcirculation.