电针对局灶性脑缺血再灌注损伤大鼠学习记忆功能的影响

Effect of Electroacupuncture on Learning and Memory Ability of Cerebral Ischemia/Reperfusion Injured Rats

目的探讨电针神庭、百会对局灶性脑缺血再灌注损伤大鼠学习记忆功能的影响及其机制。方法雄性Sprague-Dawley大鼠45只,随机分为假手术组(n=15)、模型组(n=15)和电针组(n=15)。模型组、电针组用线栓法制备局灶性脑缺血2 h再灌注损伤模型。电针组行电针干预7 d。各组在造模后3 d开始行Morris水迷宫测试。7 d后尼氏染色观察海马神经细胞变化,逆转录PCR检测Bcl-2、Bax mRNA表达。结果电针组逃避潜伏期显著低于模型组(P<0.001),穿过平台次数显著低于模型组(P=0.001)。电针组海马区细胞损伤及Bax mRNA水平降低、Bcl-2 mRNA水平提高(P<0.05)。结论电针能改善局灶性脑缺血再灌注损伤大鼠学习记忆能力,保护神经细胞,其机制可能与调节凋亡相关基因Bax、Bcl-2的表达有关。

Objective To investigate the effect of electroacuptmcmre at Shenting （DU24） and Baihui （DU20） on learning and memory ability, as well as the nerve cell and the expression of mRNA of Bcl-2 and Bax in the hippocampus of rats with cerebral ischemia/reperfusion （I/R） injury. Methods 45 male Sprague-Dawley rats were randomly diveded into sham group （n=15）, control group （n=15） and electroacupuncture group （n=15）. The latter 2 groups were modeled with middle cerebral artery occlusion for 2 h and reperfusion. The electroacupuncture group received electroacupuncture at Shenting and Baihui for 7 days. They were tested with Morris water maze, and then, their hippocampus was observed under Nissl staining and the level of Bcl-2 and Bax mRNA were determined with RT-PCR. Results The escape latency decreased （P〈0.001） and the frequence the platform was crossed increased （P=-0.001） in the electroacupuncture group compared with the model group, with less damage of the nerve cell and the expression ofBax mRNA, and more ofBcl-2 mRNA （P〈0.05）. Conclusion Electroacupuncture can prevent learning and memory ability from cerebral ischemia/reperfusion injury in rats, which may associate with adjustment of expression of apoptosis-related gene to inhibit apoptosis of nerve cell.