慢性心理应激诱导的AS兔模型血脂变化特征及其机制探讨

Change features of blood lipids in chronic psychological stress-induced atherosclerosis of rabbits and the mechanism

目的探讨慢性心理应激诱发动脉粥样硬化(AS)的可能机制。方法将160只健康雄性新西兰兔随机分为对照组、高脂组应激组、应激+高脂组各40只。对照组及应激组予普通饲料喂养,高脂组、应激+高脂组予高脂饲料喂养;应激组及应激+高脂组均予旋转、噪音、惊恐、断食、断水等刺激致心理应激制备AS模型;共16周。于制模前及制模第4、8、12、16周末测定各组血脂(包括TC、TG、LDL-c、HDL-c);分离腹主动脉组织行油红O染色,观察脂质沉积情况。结果第4、8周血脂水平应激+高脂组>高脂组>应激组(P<0.05);第12、16周末,与对照组比较,应激组HDL-c水平明显下降(P<0.05),其余指标明显升高(P<0.05);高脂组与应激组LDL-c水平无统计学差异,其余指标均明显升高(P<0.05);与高脂组比较,应激+高脂组HDL-c明显降低(P<0.05),其余指标均明显升高(P<0.05)。脂质沉积情况:应激+高脂组、高脂组、应激组分别于第4、8、12周开始有脂质沉积,第16周应激+高脂组>高脂组>应激组(P<0.05)。结论慢性心理应激诱导的AS脂质紊乱特征与高脂所致的AS的机制不同,其促进AS形成的机制可能为引起LDL-c水平升高、HDL-c降低。

Objective To investigate the clinical features of atherosclerosis （ AS） induced by chronic psychological stress （ CPS） and the possible mechanism .Methods Totally 160 New Zealand male rabbits were randomly divided into 4 groups, 40 cases in each group：the control group （group C）, CPS group, high cholesterol feed group （HCF group） and chronic stress plus high cholesterol feed group （ CPS＋HCF group ） .Rabbits in the C and CPS groups were fed with stand-ard diet;HCF and CPS＋HCF groups were fed with high-fat diet.Rabbits in the CPS and CPS＋HCF groups underwent ro-tation, noise, panic, fasting and water stimulation to induce the psychological stress in order to prepare the AS models .The experiment lasted for 16 weeks.The concentrations of TC , TG, LDL-c, HDL-c and abdominal aorta of the rabbits stained by oil red O were examined before modeling and after 4, 8, 12 and 16 weeks respectively .Results At week 4 and 8, the concentrations of TC, TG, LDL-c and HDL-c：CPS＋HCF group 〉HCF group 〉CPS group （P〈0.05）.At the end of 12th and 16th weeks, the serum HDL-c level of group CPS decreased significantly as compared with that of group C （P〈0.05）, and the rest indexes increased significantly （P〈0.05）; the serum LDL-c level had no statistical difference be-tween the CPS and HCF groups （P〉0.05）, and the other indexes decreased significantly （P〈0.05）;the serum HDL-c level of group CPS＋HCF significantly decreased as compared with that of group HCF （P〈0.05）, and the other indexes increased （P〈0.05）.The lipidoses in arterial wall of CPS ＋HCF group （after 4 weeks）, HCF group （after 8 weeks） and CPS group （after 12 weeks） were observed.After 16 weeks, HCF group exhibited more lipidoses in arterial wall than CPS group and CPS＋HCF group exhibited more lipidoses than HCF group （ all P〈0.05） .Conclusion The features of blood lipids metabolic disorders of AS induced by CPS are different from that induced by HCF .CPS maybe contributes to AS by＆amp;nbsp;increasing LDL-c level and decreasing HDL-c level.