载脂蛋白B基因多态性与激素性股骨头坏死筋脉淤滞证的相关性

Relationship between apolipoprotein B gene polymorphism and tendon-vessel stagnation syndrome of steroid induced osteonecrosis of the femoral head

目的探讨载脂蛋白B(Apo B)基因多态性与激素性股骨头坏死(SONFH)筋脉淤滞证的相关性。方法SONFH 108例患者,中医分型为筋脉瘀滞证64例(筋脉瘀滞组),肝肾亏虚证44例(肝肾亏虚组),另选择因内科疾病使用肾上腺皮质激素治疗半年后未发生SONFH的患者98例为对照组。提取三组血样品DNA,采用直接测序法及PCR-LDR法检测ApoB基因XbaⅠ(rs693)和EcoRⅠ(rs1042031)位点的单核苷酸多态性。结果 XbaⅠ位点共检出X-X-和X+X-两种基因型,筋脉瘀滞组X+X-基因型及X+等位基因频率均高于肝肾亏虚组及对照组(P均<0.05)。EcoRⅠ位点共检出E+E+和E+E-两种基因型,筋脉瘀滞组E+E-基因型及E-等位基因频率均高于肝肾亏虚组及对照组(P均<0.05)。肝肾亏虚组XbaⅠ、EcoRⅠ基因型与等位基因频率分布与对照组相比,差异无统计学意义。结论 ApoB基因XbaⅠ位点的X+X-基因型、X+等位基因及EcoRⅠ位点的E+E-基因型、E-等位基因与SONFH筋脉瘀滞证的发生有关。

Objective To study the relationship between apolipoprotein B （ ApoB） gene polymorphism and the tendon-vessel stagnation of steroid induced osteonecrosis of the femoral head （ SONFH） .Methods A total of 108 patients with SON-FH were included in this study , including 64 patients with tendon-vessel stagnation syndrome of TCM （ tendon-vessel stagna-tion syndrome group ） and 44 patients with the deficiency of liver and kidney syndrome of TCM （ deficiency of liver and kidney syndrome group ） .There were 98 patients who adopt adrenal cortical hormone for treatment in 6 months without SONFH col-lected as control group .DNA of each group were extracted from the blood sample .The direct sequencing polymerase chain re-action and ligase detection reaction （PCR-LDR） technique were used to analyze XbaⅠ（rs693） and EcoRⅠ（rs1042031） single-nucleotide polymorphism of ApoB gene .Results Two gene type, X-X-and X＋X-, were detected in XbaⅠsite.The amounts of X＋X-genotype and X＋allele frequencies of XbaⅠpolymorphism in tendon-vessel stagnation syndrome group were higher than those in deficiency of liver and kidney syndrome group （all P〈0.05）.Two gene type , which were E＋E＋and E＋E-, were detected in EcoRⅠsite.The amounts of E＋E-genotype and E-allele frequencies of EcoRⅠpolymorphism in tendon-vessel stagnation syndrome group were higher than those in deficiency of liver and kidney syndrome group （all P〈0.05）. However , the difference between the deficiency of liver and kidney syndrome and the control group showed no significance （ P〉0.05）.Conclusion The XbaⅠX＋X-genotype, XbaⅠX＋allele frequencies, EcoRⅠE＋E-genotype and EcoRⅠE-al-lele frequencies may be related to the tendon-vessel stagnation syndrome of SONFH .