多肽P161联合顺铂对多种肿瘤细胞增殖的抑制作用

Inhibitory Effect of Polypeptide P161 Combined with Cisplatin on Proliferation of Multiple Cancer Cells

目的体外检测多肽P161联合顺铂(DDP)对多种肿瘤细胞增殖的抑制效应。方法采用四甲基偶氮唑盐(MTT)法检测不同浓度DDP、P161单用及联合用药对人肝癌HepG2、人结肠癌HT29、人前列腺癌IE8、人胰腺癌Panc-1及小鼠乳腺癌MA-782的生长抑制率。结果 DDP和P161对多种肿瘤细胞均有剂量依赖性的抗增殖作用。联合给药组表现为协同效应,与DDP组比较差异有统计学意义(P<0.05),在相同的抑癌效果下可降低DDP的使用量。且在相同浓度梯度下,联合用药组对Panc-1的抑制效果较差,而对MA-782效果最好。结论 P161可以促进肿瘤细胞对DDP的敏感性,二者联合用药可降低DDP有效治疗浓度。

Objective In vitro detection of anti-proliferative effects of P161 combined with cisplatin（ DDP） on multiple cancer cells. Methods Growth inhibition rates of HepG2,HT29,IE8,Panc-1 and MA-782 treated by different concentrations of DDP,P161 and P161 combined with DDP were determined by MTT assay. Results DDP and P161 dosedependently inhibited proliferation of multiple tumor cells. A synergistic effect was found in DDP combined with P161 and there was a significant difference in the effect between DDP combined with P161 and DDP alone（ P 〈0. 05）. DDP dose could be decreased to reach the same inhibitory effect. In the same concentration gradient of DDP combined with P161,the inhibition rate of Panc-1 was low and that of MA-782 was high. Conclusion P161 can increase the sensitivity of tumor cells to DDP. The combination of P161 and DDP can reduce the effective therapeutic concentration of DDP.