姜辣素对小鼠B16细胞黑素合成的影响

Effects of Gingerol on Melanogenesis of B16 Melanoma Cells

目的探讨姜辣素对小鼠B16细胞黑素合成的影响。方法黑素瘤细胞分别加入12.5,25.0,50.0,100.0,200.0μmol·L-1姜辣素和阳性对照药物氢醌培养,达尔伯克改良伊格尔培养液(DMEM)作为空白对照组。采用四噻唑蓝(MTT)比色法测定细胞增殖,比色法测定酪氨酸酶活性和黑素含量。结果与空白对照组比较,含姜辣素的给药组均能够明显抑制小鼠B16细胞增殖(P<0.05或P<0.01),且浓度为200.0μmol·L-1时,抑制率>48%。与空白对照组比较,各浓度姜辣素均能够显著降低酪氨酸酶活性(P<0.05或P<0.01),姜辣素的浓度为200.0μmol·L-1时,对酪氨酸酶的活性的抑制率可达50%。与空白对照组比较,各浓度的姜辣素和氢醌均能够显著降低黑素含量(P<0.05或P<0.01),但不呈剂量依赖性,浓度>25.0μmol·L-1时,抑制黑素含量的水平基本无变化。结论姜辣素能有效抑制黑素细胞增殖和降低酪氨酸酶活性和黑素含量,从而减少黑素合成。

Objective To study the effects of the gingerol on the melanogenesis in melanoma B16 cells. Methods Melanoma cells were cultured with gingerol at 12. 5,25. 0,50. 0,100. 0,200. 0 μmol · L^-1and positive control drug hydroquinone,respectively,using Dulbecco＇s modified eagle＇s medium（DMEM） as the blank control group. The cell proliferation was measured by methyl thiazolyltet tetrazolium（ MTT） colorimetric assay. The tyrosinase activity and melanin content were measured by colorimetry assay. Results Gingerol at different concentrations had inhibitory effect on B16 cell proliferation compared with the blank control group（ P〈 0. 05 or P 〈0. 01）,the inhibition rate being more than 48% at the dosage of200. 0 μmol·L^-1. Tyrosinase activity was inhibited significantly compared with blank control group（P 〈0. 05 or P〈 0. 01）,the inhibition rate being up to 50% at the dosage of 200 μmol·^L-1. Melanin content was also decreased at all levels of gingerol compared with blank control group（P 〈0. 05 or P 〈0. 01）,but not in a dose-dependent manner. The inhibition rate of melanin content reached the plateau at gingerol levels greater than 25. 0 μmol · L^-1. Conclusion Gingerol can inhibit the cell proliferation,tyrosinase activity and decrease melanin synthesis in certain range of concentrations.