鞣花酸对骨髓瘤SP2/0细胞的作用

Effect of Ellagic Acid on Myeloma SP2/0 Cells

目的探讨五倍子提取物鞣花酸抗骨髓瘤的生物学活性及相关机制。方法 20,40和60μg·mL-1鞣花酸体外孵育骨髓瘤SP2/0细胞株,采用细胞形态学、细胞增殖实验和流式细胞仪检测鞣花酸对SP2/0细胞株增殖、凋亡和细胞周期的影响,通过Western blotting技术检测肿瘤细胞增殖与凋亡相关基因COX-2的表达。结果 20,40,60μg·mL-1鞣花酸孵育骨髓瘤SP2/0细胞株48 h后,细胞周期阻滞于G1期,G1期细胞百分率分别为(55.21±3.01)%,(64.48±0.43)%,(75.10±2.46)%,与对照组(34.04±1.74)%比较,差异有统计学意义(P<0.01)。20,40和60μg·mL-1鞣花酸细胞抑制率分别为(21.18±5.92)%,(44.58±3.43)%和(70.15±2.90)%,与对照组比较,差异有统计学意义(P<0.01)。细胞早期凋亡率分别为(9.60±0.56)%,(19.30±1.51)%和(35.10±5.26)%,与对照组(3.23±0.85)%比较,差异有统计学意义(P<0.01),随药物浓度的增加COX-2的表达逐渐下降。结论鞣花酸能够抑制骨髓瘤SP2/0细胞增殖,促进其凋亡。

Objective To investigate the effect of ellagic acid extracted from gallnut on multiple myeloma SP2 /0 cell line and related mechanisms. Methods Multiple myeloma SP2 /0 cell line was treated for 48 h with different concentrations of ellagic acid in vitro. Cell morphology,proliferation,apoptosis and cell cycle were analyzed with microscope,MTT experiment and flow cytometry,respectively. Tumor cell proliferation and apoptosis-related gene expression of COX-2 were detected by Western blotting. Results Cell cycle was arrested at the G1 phase 48 h after treatment with ellagic acid,the cell in G1were（55. 21 ±3. 01）%,（64. 48 ± 0. 43）%,（75. 10 ± 2. 46）%,respectively,with significant difference as compared with control group[（34.04 ±1.74）%,P 〈0. 01]. Cell suppression rate（21. 18 ± 5. 92）%,（44. 58 ± 3. 43）% and（70. 15 ± 2. 90）%,respectively,in 20,40 and 60 μg·mL^-1ellagic acid treatment groups. Compared with the control group,the differences were significant（P 〈0. 01）. Cell apoptosis rate was（9. 60 ± 0. 56）%,（19. 30 ± 1. 51）% and（35. 10 ± 5. 26）%,respectively,in20,40 and 60 μg·mL^-1ellagic acid treatment groups,with significant differences as compared with the control group[（3. 23 ±0. 85）%,P 〈0. 01]. With the increase of drug concentration,COX-2 expression was decreased. Conclusion Ellagic acid can inhibit myeloma SP2 /0 cell proliferation and promote apoptosis.