摘要
目的 探讨 β -胡萝卜素拮抗三氧化二镍的细胞毒作用及其机理。 方法 采用体外细胞培养方法 ,观察 β -胡萝卜素对染镍肺泡巨噬细胞膜脂质过氧化产物丙二醛 (MDA)及超氧化物歧化酶 (SOD)活性的影响。结果 在体外染镍肺泡巨噬细胞培养过程中加入不同浓度 β -胡萝卜素 (2 5 ,5 0和 10 0 μmol/L)可提高肺泡巨噬细胞内SOD活性 ,呈剂量 -反应关系。维生素E能显著抑制MDA生成量 ,其中以 5 0 μmol/L和 10 0 μmol/L浓度组作用最为明显。 结论 镍可致细胞脂质过氧化 ,β -胡萝卜素具有拮抗镍所致细胞毒性的作用 ,可能与其抗氧化功能有关。
Objective To investigate the effects and mechanism of β-carotene on the injury of alveolar macrophage exposed to Ni 2O 3.Methods The alveolar macrophage was cultured in vitro with exposure to Ni 2O 3 and the detection of malondialdehyde and activity of antioxidant enzyme in alveolar macrophage were carried out.Results The results of adding different dose of β-carotene(25,50,100μmol/L) could increase the activity of superoxide dismutase in alveolar macrophage with an dose-dependent manner.Vitamin E could inhibit the production of malondialdehyde apparently.Both of the β-carotene 50μmol/L group and 100μmol/L group had dramatic inhibitory effect.Conclusion Ni 2O 3 could induce over oxidation of cytoplasm β-carotene could inhibit the cytotoxity of Ni 2O 3,which probably due to its antioxidant property.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2002年第8期898-899,共2页
Chinese Journal of Public Health
基金
铁道部科技基金资助项目J2 0 0 0Z1 0 8