铅对大鼠肾脏一氧化氮合酶、一氧化氮影响的研究

Effects of Lead on Changes of NO.NOS in Rat Kidney

目的 探讨慢性染铅过程中大鼠肾脏一氧化氮合酶 (NOS)、一氧化氮 (NO)的变化。方法 Wistar大鼠经饮水加 0 0、18 4、184 0mg/L醋酸铅 (PbAc)方法染毒。结果 高剂量组大鼠肾脏NOS活力 30d后低于对照组 (P <0 0 5 )。低剂量染铅组大鼠肾脏NOS活力 6 0d后低于对照组 (P <0 0 5 )。低剂量染铅组肾脏NO含量在各时间点上均有降低趋势 ,但差异无统计学意义 (P >0 0 5 )。高剂量组大鼠肾脏NO含量在 6 0d、90d后低于对照组 (P <0 0 5 )。结论 铅可导致肾脏NOS活力降低 ,NO含量降低 ,可能是铅对肾脏产生细胞毒作用的机制。

Objective To study the effect of chronic lead exposure on the changes of nitric oxide synthase(NOS) activity and nitric oxide(NO) content in kidney.Methods Wistar rats were exposed to lead from drinking 0,18.4 and 184mg/L lead acetate solution.Results Compared with control group,kidney NOS activity in high-dose lead exposed groups was significantly decreased after 30 days exposure( P <0 05).NOS activity in low-dose lead exposure groups increased,but show no significant difference( P >0 05).NO content in kidney were markedly decreased after 90 days exposure in low-dose group and 60 days in high-dose group( P <0 05).Conclusion NOS activity and NO content in kidney were significantly decreased during chronic lead exposure.The decrease of NOS activity and NO content resulted from lead exposure may be correlated with the neural poisoning of kidney.