杠柳甲醇提取物杀虫活性成份分离及测定的研究

Periploca Methyl Alcohol Extraction Insect Disinfestation Active Ingredient Separation and Determination Research

采用硅胶柱层析对陕北杀虫植物杠柳根皮的甲醇提取物进行分离,得到杀虫成分A-4,经GC-MS分析,A-4的化学成分主要有:(9E,12E)-十八二烯酸(34.48%),棕榈酸(17.97%),9Z,12Z-十八碳二烯酸甲酯(3.18%)和棕榈酸甲酯(1.34%);采用"Y"形嗅觉测试仪测定了其对红火蚁工蚁的趋避活性。结果显示:当A-4浓度为0.01 g/mL时,对红火蚁大工蚁有显著的驱避活性(P=0.0013)。

The plant periploca root skin was separated by gel column chromatographic. The result showed that con-stituent of A-4 exhibited significant（P= 0. 0013）repellent activity at 0. 01g/mL by using Y-tube olfactometer. The major compounds of A-4 fraction were（9E,12E）-octadeca -9,12 -dienoic acid（34. 48%）,palmitic acid（17. 97%）,（Z,Z）-9,12-octadecadienoic acid-methyl ester（3. 18%）and methyl palmitate（1. 34%） by GC-MS analysis.