甲基叔丁基醚对整体染毒小鼠的遗传毒性研究

Study on Vivo Genotoxicity of Methyl Tertiary-Butyl Ether in Mice

目的 探讨国产甲基叔丁基醚的遗传毒性作用。方法 对小鼠经呼吸道染毒 2 0天 ,染毒剂量分别为 10 8、14 4 0、4 96 8mg/m3 ,分别进行单细胞凝胶电泳试验、DNA交联试验和微核试验。结果 甲基叔丁基醚可引起整体染毒小鼠肝、肾、肺细胞的DNA单链断裂 ,可引起 4 96 8mg/m3 剂量组动物微核率的增加 ,不引起肝细胞DNA的交联。结论 甲基叔丁基醚在DNA水平和染色体水平有一定的遗传毒性作用。

Objective To explore the genotoxicity of domestic produced Methyl Tertiary-Butyl Ether (MTBE).Methods The mice were administered MTBE by inhalation at 108,1 440 or 4 968mg/m\+3 for 20 consecutive days. The genotoxicity of MTBE in mice was detected by single cell gel electrophoresis assay, DNA cross-links test and mouse bone marrow micronucleus test.Results MTBE could cause DNA single strand breaks in mice hepatocytes, kidney cells and lung cells.It could increase the frequency of mice bone marrow micronucleus at 4 968mg/m\+3.MTBE could not induce DNA cross-links of hepatocytes in vivo.Conclusion MTBE produced in the domestic had some genotoxicity at DNA level and chromosome level.