解偶联蛋白1、2和3基因在中国西门塔尔牛组织器官中的表达水平及其与胴体品质关系分析

Analyze the ucp1,ucp2,ucp3 Genes Expression Level in Tissues /Organs and the Relationship with the Carcass Traits in Chinese Simmental Cattle

为获得中国西门塔尔牛解偶联蛋白(Uncoupling proteins,UCPs)ucp1、ucp2、ucp3基因的组织表达谱,揭示该基因与能量代谢的关系,探索调控肉牛脂肪沉积的关键基因表达规律,筛选影响肉牛肥育性状的候选基因。以20头中国西门塔尔牛为试验材料,提取其15种组织(肝脏、淋巴、肋间肉、肩肉、脾脏、肾脏、背最长肌、肺、心脏、肠脂、网脂、睾丸、小肠、胰脏、背膘)总RNA,设计合成ucp1、ucp2、ucp3基因的引物,以牛β-actin基因为内参,利用实时荧光定量PCR技术,检测ucp1、ucp2、ucp3基因的mRNA在以上各组织中的表达。结果表明,ucp1、ucp2、ucp3基因在睾丸、小肠、心脏、胰腺等组织、器官中均有不同程度的表达,ucp1在背膘中高表达,胰脏、小肠、睾丸、网脂、肠脂、心脏中度表达,在其他组织中低表达;ucp2在睾丸中高表达,肺脏、肝脏、背膘中度表达,在其他组织中低表达;ucp3在背最长肌、肋间肉、肩肉中高表达,肺脏中度表达,在其他组织中低表达。经相关分析表明:眼肉中ucp3基因的表达量与肌内脂肪和背膘厚关系不大,而与眼肌面积呈正相关(r=0.788)。结论:中国西门塔尔牛ucp1、ucp2、ucp3基因的表达,具有组织特异性;ucp1、ucp2、ucp3在不同组织的表达差异,可能与其在不同组织中的功能有关;ucp3基因可作为与胴体性状相关的候选基因。

The objective is to obtain expression profile of Chinese Simmental cattle ucp1,ucp2,ucp3 genes,to reveals the relationship between the ucp1,ucp2,ucp3 gene and energy metabolism ,to explore the expression rule of key gene of fat deposition ,and to screen the candidate gene affecting fat deposition .Total RNA were extracted from fifteen tissues（liver,lymph,intercostals muscle,shoulder muscle,spleen,kidney,rib eye muscle,lung,heart,gut fat, greater omental fat,testicle,small intestine,pancreas and backfat）of twenty animals from Chinese Simmental cattle . The primers of ucp1,ucp2,ucp3 genes were designed and synthesized ,and the data were normalized using the β-ac-tin gene as internal control .The specific expression of ucp1,ucp2,ucp3 genes in different tissues were investigated by real-time quantitative PCR.There were different degree expression of ucp1,ucp2,ucp3 genes mRNA in the tissues and organs of testicle ,small intestine ,heart,pancreas and so on .The expression of ucp1 was highest in the backfat , middle in the pancreas,small intestine,testicle,greater omental fat,gut fat,and heart,and lowest in the other tis-sues.The highest level expression of ucp2 was detected in Testicle ,and there were also higher expression of ucp2 in the lung,liver,and backfat than in other tissues .The highest level expression of ucp3 was in rib eye muscle,Inter-costal muscle ,and shoulder muscle ,middle in the lung ,and the lowest in other tissues .Correlation analyses showed that the expression level of ucp3 gene in rib eye had no significant relation with intramuscular fat and backfat thick-ness,but had positive relation to eye lean area（r=0.788）.It is concluded that the expression level of ucp1,ucp2, ucp3 genes had tissue specific might be related to the various functions on different tissues of cattle .The ucp3 gene can be used as a candidate gene related to carcass traits .