摘要
为进一步挖掘白刺属植物果实中花青素成分,以采自内蒙古两个地点的西伯利亚白刺果实为材料,利用高效液相色谱(HPLC-DAD)技术,建立了定性定量分析西伯利亚白刺果实中花青素的方法,同时结合离子阱多级质谱(MSn)技术,从西伯利亚白刺果实甲醇提取物中鉴定出12种花青素组分,分别为:矢车菊素3-槐糖苷、矢车菊素3-戊糖苷、矮牵牛素3-芸香糖-葡萄糖苷、芍药素3-槐糖苷、矮牵牛素3-鼠李糖苷、锦葵素3-阿拉伯糖苷、芍药素3-己糖苷、矢车菊素3-顺式-香豆酰二葡萄糖苷、矢车菊素3-反式-香豆酰二葡萄糖苷、天竺葵素3-香豆酰二葡萄糖苷、芍药素3-丙二酰葡萄糖苷、矮牵牛素3-香豆酰葡萄糖苷。利用所建立的方法,分析并比较了3个不同成熟阶段和2个采集点样品中花青素组成和含量的差异。
In the present study ,an HPLC method was established for qualitative and quantitative analysis of the fruits of Nitraria sibirica based on the HPLC-DAD/TRAP-MS technique .The chemical structure of 12 anthocyanin components were identified , including：Cyanidin 3-O-sophoroside , Cyanidin 3-O-hexose , Cyanidin 3-O-（ cis-p-cou-maroyl）-diglucoside,Cyanidin 3-O-（trans-p-coumaroyl）-diglucoside,Petunidin 3-O-rutinoside-glucose,Petunidin 3-O-rhamnoside,Petunidin 3-O-（p-coumaroyl）-glucoside,Peonidin 3-O-hexose,Peonidin 3-O-sophoroside,Peonidin 3-O-malonyl-glucoside,Pelargonidin 3-O-（p-coumaroyl）-diglucoside and Malvidin 3-O-arabinose.In addition,the content of the anthocyanin components were also determined .It provides the scientific basis for the further exploita-tion and utilization of anthocyanins in Nitraria sibirica.
出处
《华北农学报》
CSCD
北大核心
2014年第4期130-134,共5页
Acta Agriculturae Boreali-Sinica
基金
国家自然科学基金项目(31140020)
内蒙古自然科学基金重大项目(2012ZD05)
