类猪圆环病毒 P1转录的 Northern Blot 分析

Transcriptional Analysis of Porcine Circovirus-like Virus P1 by Northern Blot

为鉴定类猪圆环病毒P1的转录本,采用地高辛标记的RNA探针,通过Northern Blot方法,对转染PK15细胞的类猪圆环病毒P1进行了RNA转录分析。结果表明,Northern杂交可检测到2个RNA转录本,包括正链上ORF3的RNA和负链上ORF1的RNA。同时,ORF3 RNA表达时间比ORF1的短,且丰度也大大低于ORF1。类猪圆环病毒P1至少存在ORF1和ORF3 2个转录本。

The purpose of this study is to identify transcripts of porcine circovirus-like virus P1.In this work, the RNAs of porcine circovirus-like virus P1 synthesized in PK15 cells were characterized by Northern Blot analysis using the DIG-labeled riboprobes .The results showed two RNAs were detected .They included ORF3 RNA and ORF1 RNA.Meanwhile,ORF1 RNA had both more abundant and longer expression than those of ORF3 RNA. Northern Blot analysis indicated that at least two viral-specific RNAs were synthesized in P 1.infected PK15 cells.