固定化漆酶在染料脱色中的应用

Application of immobilized laccase in dye decolorization

为了使解淀粉芽孢杆菌LC03的芽孢漆酶对染料废水有更好的脱色作用,将其固定于海藻酸钙的凝胶中,测定其最适温度和最适pH及操作稳定性,并对RBBR、靛红(IC)、活性黑(RB5)、结晶紫(CV)等4种单染料进行脱色;通过加入漆酶介体,研究固定化漆酶-介体系统对单染料及模拟染料废水脱色的影响。结果表明:固定化漆酶最适温度65℃,最适pH为6.2,重复利用10次酶活还在80%以上;固定化小球对染料CV脱色作用尤为明显,脱色1 h后脱色率就达到80%以上,对其他3种染料也有一定的脱色作用;漆酶介体对染料的脱色均有促进作用,对染料IC、RBBR和RB5的促进作用尤为明显,且随着介体浓度的升高脱色率增大;固定化小球提高了漆酶的重复利用率,增加了漆酶的稳定性,在酸性、碱性、中性条件下,对单染料和模拟染料废水都有很好的脱色效果。

In order to obtain a good decolorization efficiency of dye wastewater,we fixed spore laccase from Bacillus amyloliquefaciens LC03 in seaweed acid calcium gel and determined its optimum temperature and pH and its operational stability. Then we studied the decolorization of four single dyes,including remazol brilliant blue R（ RBBR）,reactive black5,indigo carmine and crystal violet,and investigated the effects of immobilized laccase-mediators with laccase mediators added on single dye to stimulate dye wastewater. Experiments showed that the optimum temperature was 65 ℃,the optimum pH was 6. 2 and after 10 cycles of operation the immobilized enzyme remained over 80% of its initial activity. The immobilized pellets had a significant effect on decolorization of CV and more than 80% of CV was decolorized after 1hour,while it also played some role in the decolorization of other three single dyes. Laccase mediators had a promoting effect on the decolorization of dyes especially on the IC,RBBR and RB5 and with the mediators concentration rising,the rate of decolorization increased. The immobilized pellets enhanced laccase repeated utilization ratio and increased the stability of laccase. And under the acid,alkaline,and neutral conditions,immobilizod laccase mediators had a good decolorizing effect on single dye and simulated dye wastewater which indicating potential application of this immobilized laccase-mediators in dye wastewater treatment.