三十烷醇对大鼠离体心脏缺血再灌注损伤的影响

Effect of Triacontanol on Myocardial Ischemia-reperfusion Injury in Isolated Rat Heart

【目的】观察三十烷醇（triacontanol ，TRIA）对心肌缺血再灌注损伤（IRI）的影响。【方法】采用Langendorff灌流装置建立大鼠离体心脏IRI模型。将大鼠随机分为正常组、缺血再灌注（IR）组、不同剂量T RIA＋IR组及T RIA （2．5μg/mL）组。正常组全心持续灌流90 min；IR组平衡灌注30 min ，缺血30 min后再灌注30 min；TRIA＋IR组在缺血前5 min分别给予（25，15，5，2．5）μg/mL TRIA之后处理同IR组；TRIA组平衡灌注30 min后给药5 min ，之后持续灌流30 min。观测各组心率（HR）、冠脉流量（CF）、左室内压（LVP）和左室内压变化最大速率（± dp/dtmax），测定冠脉流出液中肌酸激酶（CK）释放量。【结果】与正常组比较，IR组引起明显的心功能损伤，表现为再灌注期间 HR、CF、LVP、± dp/dtmax降低以及CK释放量增加；心脏灌注不同剂量TRIA（25，15，5，2．5μg/mL）5 min可加重IRI所致心功能损伤，甚至心脏停跳，表现为LVP、± dp/dtmax进一步降低，CF显著减少，HR减慢以及CK释放量增加；单独灌注TRIA（2．5μg/mL）对大鼠心功能也有明显抑制作用，抑制作用持续30 min不能恢复。【结论】离体大鼠心脏灌流 T RIA可抑制心功能及加重心肌IR后心功能的损伤。

[Objective]To explore the effect of triacontanol（TRIA） on myocardial ischemia-reperfusion injury（IR） in isolated rat cardiac IR model .[Methods]Langendorff perfusion device was used to establish rat isolated heart IR model .All rats were randomly divided into normal group ,different dose TRIA＋IR group and TRIA（2 .5 g/ml） group .The normal group was given whole heart continuous perfusion for 90min .The IR group was given the balance for 30min and then reperfusion for 30min after 30min-ischemia .The TRIA-IR group was given 25 ,15 ,5 and 2 .52 .5 g/ml TRIA at 5min before ischemia and then the same treatment as IR group .The TRIA group was given the medication for 5min after 30min-balance and then continuous perfusion for 30min .Heart rate（HR） ,coronary flow （CF） ,left ventricular pressure （LVP） and maximum velocity of LVP variation（dp/dtmax ） were observed .The level of creatine kinase（CK） in coronary effluent was measured .[Results]Compared with normal group ,IR could induce the obvious heart dysfunction as shown by a decrease in HR ,CF ,LVP and dp/dtmax and an increase in CK level .Heart perfusion with different doses of TRIA （25 , 15 ,5 and 2 .5 g/mL） for 5min aggravated IR-induced cardiac dysfunction and even heart arrest as shown by a further decrease in HR ,CF ,LVP and dp/dtmax and an increase in CK level .TRIA（2 .5 g/mL） alone also inhibited rat cardiac function which was not recovered for continuous 30min .[Conclusion]TRIA can inhibit cardiac function and aggravate myocardial IR injury in Langendorff rat .