中药成分对新城疫强毒感染DF1能力的影响

Effects of Chinese medicinal ingredient on the ability of Newcastle disease virulent strain infecting DF1

将安全浓度范围内5种浓度的黄芩苷、氧化苦参碱和三七总皂苷等10种中药成分与鸡新城疫强毒(F48E8)分别以3种方式加入到单层鸡胚成纤维细胞(DF1)中,然后用MTT法测定OD值,以评价各中药成分的抗病毒感染能力。结果黄芩苷、氧化苦参碱、三七总皂苷、菊花总黄酮抑制作用较强。这些单个中药成分为中药抗病毒复方研究提供了理论基础。

10 Chinese medicinal ingredients all in 5 different safety concentrations,and NDV virulent strain（F48E8）were added into DF1 in three ways respectively,then the OD value was measured with MTT method to evaluate the ability of Chinese medicine against virus infection. The results showed that baicalin,oxymatrine,Panax Notoginsenosidum and chrysanthemum flavone inhibited stronger which provide dtheoretical basis for researching of traditional Chinese medicine compounds.