摘要
目的:构建并观察 CD46-siRNA 对人乳腺癌 MCF-7细胞增殖、侵袭及转移能力的影响。方法构建 pEGFP-C1-CD46 siRNA 并转染 MCF-7细胞,检测 CD46 mRNA 及蛋白的表达水平变化,观察 MCF-7/CD46-siRNA 细胞侵袭及迁移能力变化。结果与对照组及乱序组相比,沉默组MCF-7细胞 CD46 mRNA 相对表达量显著降低(P <0.05),CD46蛋白表达也显著被抑制。随着时间的延长,对照组和乱序组 MCF-7细胞增殖活力逐渐升高,而沉默组 MCF-7细胞增殖活力升高不明显。第3、4天时,三组细胞增殖活力差异有统计学意义,且沉默组均显著低于其他两组(P <0.05)。对照组及乱序组 MCF-7细胞侵袭及迁移计数显著高于沉默组(P <0.05)。结论pEGFP-C1-CD46 siRNA 表达载体能够有效抑制乳腺癌 MCF-7细胞 CD46基因及蛋白表达,可以降低乳腺癌细胞增殖活力、侵袭及迁移能力。
Objective To establish and observe the effects of CD46-siRNA on proliferation,in-vasion and metastasis of MCF-7 human breast cancer cell.Methods The pEGFP-C1-CD46 siRNA was established and transfected into the MCF-7 cell.Changes in CD46 mRNA and protein expression were test-ed and variation of invasion and migration capacity in MCF-7 /CD46-siRNA cell was also observed.Results Compared with the control group and scrambled group,the expression of CD46 mRNA was significantly decreased(P 〈0.05)and CD46 protein expression was also suppressed in the MCF-7 cells of the silence group.Proliferation activity of MCF-7 cell in the control group and scrambled group was gradually in-creased with the time,but this phenomenon was not obvious in the silence group.At the 3th and 4th day af-ter transcription,differences in proliferation activity were significant among the groups,and activity in the silent group was significantly lower than the other two groups(P 〈0.05).Counts in invasion and migration of MCF-7 cell in the control group and scrambled group were significantly higher than the silent group (P 〈0.05 ).Conclusion The expression vector of pEGFP-C1-CD46 siRNA can suppress the CD46 mRNA and protein expression in MCF-7 human breast cancer cell and reduce the capacity in proliferation activity,invasion and migration.
出处
《临床外科杂志》
2014年第8期564-567,共4页
Journal of Clinical Surgery
基金
河北省沧州市科技计划项目