中山地区人群C5L2基因C698T与妊娠期糖尿病相关性研究

Studies on polymorphism of C5L2 gene in GDM patients in Zhongshan region

目的研究促酰化蛋白(acylation-stimulating protein,ASP)的功能受体C5L2基因的SNP位点C698T多态性与中山地区人群妊娠期糖尿病(GDM)的相关性。方法本研究采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)对来自2012年2013年中山博爱医院共285例孕妇检测C5L2基因多态性,评估其与GDM的相关性。其中GDM组174例,血糖正常103例设为对照组。GDM组和对照组基因型分布运用logistic回归模型分析。GDM组相关表型运用多重回归模型分析。结果C5L2基因C698T杂合子基因型在GDM组和对照组的分布频率分别为0.6%和1.9%,差异不具有统计学意义(校正χ2=0.213,P=0.6>0.05)。基因型TT在GDM组和对照组均未发现。结论在中山地区人群中,C5L2基因C698T基因多态性与GDM的发生可能不具有相关性。

Objective： This study aimed to examine possible genetic effects of C5L2 gene polymorphisms （C698T） on the risk of GDM of Zhongshan city population. Methods： C5L2 gene 698C〉T variant from 185 patients with GDM and 100 healthy as control subjects was detected by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method. The genotype distributions between GDM and controls were analyzed using logistic regression models. In addition, GDM-related phenotypes were analyzed using multiple regression models. Result： Heterozygote carders of the C698T genotype were no different between GDM and controls （X2=0.213, P=0.644〉0.05） . We have not found TT genotype in GDM or control. Conclusion： The CT genotype of C5L2 gene might not be a risk factor of GDM in Zhongshan population.