摘要
目的探讨小儿肾母细胞瘤组织中E2F3的表达与肾母细胞瘤临床分期的相关性。方法利用免疫组织化学方法检测26例肾母细胞瘤组织中E2F3阳性细胞的百分比、积分光密度及E2F3 mRNA的表达,比较采用t检验及方差分析,P<0.05有统计学意义。结果 E2F3在所有肾母细胞瘤组织中均过度表达,在肾母细胞瘤侵蚀性强时,E2F3蛋白及mRNA的表达水平较高。结论 E2F3在小儿肾母细胞瘤组织中过度表达,E2F3表达水平与肾母细胞瘤临床分期密切相关。
Objective： The present study was undertaken to examine the expression of E2F3 and investigate its relevance in clinical stages of pediatric Wilms＇ tumors. Methods： The study included 26 cases of Wilms＇ tumors. The samples underwent immunohistochemical staining for E2F3 protein expression by measuring the percentage of E2F3-positive cells and integrated optical density, and E2F3 mRNA expression. Comparisons were made using a t-test or a one-way analysis of variance,“P〈0.05” was used for statistical significance. Results： The expression of E2F3 protein and mRNA was detectable in all the Wilms＇ tumor samples with big variations. Wilms＇ tumors with aggressive features, such as higher stage, expressed higher levels of E2F3 protein and mRNA. Conclusion： The preliminary data indicate that E2F3 is frequently expressed in pediatric Wilms＇ tumors examined in the present study. The expression levels of E2F3 correlates with clinical stages.
出处
《中国优生与遗传杂志》
2014年第9期38-41,共4页
Chinese Journal of Birth Health & Heredity
基金
黑龙江省自然科学基金(D201135)
黑龙江省教育厅科学技术研究项目(12511244)
