密度梯度联合上游法在宫腔内人工授精中的应用

The application of density gradient centrifugation combined with swim-up method in IUI

目的探讨在夫精宫腔内人工授精(IUI)中应用不同方法优化处理精液后的临床效果。方法 582个IUI周期分为3组,分别采用上游法、密度梯度法、密度梯度联合上游法三种方法处理精液,比较三种方法的精子处理效果及临床结局。结果密度梯度法处理后精子密度高于上游法和密度梯度联合上游法(P<0.05),精子活率低于上游法和密度梯度联合上游法(P<0.05)。三组妊娠结局无显著差异(P>0.05)。结论宫腔内人工授精中根据精液质量选择合适的优化精子方法,可达到较好妊娠率。

Objective： To discuss clinical effect of different optimized methods of separating high-quality sperm in artificial insemination-husband. Method： 582 IUI cycles was divided into 3 groups using different method： swim-up method, density gradient eentrifugation and density gradient centrifugation combined with swim-up method. We compare the sperm quality after treatment and clinical outcome. Result： The density of the sperm after density gradient centrifugation was higher than that of swim- up method and density gradient centrifugation combined with swim-up method, while the motility rate after density gradient eentrifugation was lower than that of the other groups. There was no significant difference in pregnancy outcome between groups. Conclusion： In order to achieve better pregnancy rate, We can choose different methods of separating high-quality sperm in IUI according to sperm quality.