正交试验设计优化消瘰颗粒剂醇提取工艺

Optimization of Ethanol Extraction Technology for Xiaoluo Granules by Orthogonal Experiment

目的:优化消瘰颗粒剂的醇提取工艺。方法:采用正交试验方法对处方中10味中药醇溶性成分进行提取研究。以黄芩苷含量和干浸膏得率两者的加权和为考察指标,选择正交表L9(34)对影响醇提取效率的四因素三水平进行考察。结果:醇溶性成分提取最优条件为:取10味中药先用10倍量50%乙醇浸泡45 min,再加热回流提取3次,每次1.5 h。结论:本法可最大限度提取处方中10味中药的醇溶性成分,可作为消瘰颗粒剂的醇提取工艺。

Objective： To optimize the ethanol extraction technology for Xiaoluo granules. Methods： The extraction technology for the ethanol dissolvable ingredients in 10 kinds of medicinal materials was optimized by orthogonal experiment. The weighted sum of baicalin content and the dry extract rate as the index, 4 influencing factors with 3 levels each were optimized by L9 （ 3^4 ） orthogonal table. Results： The optimal ethanol extraction conditions were as follows： the medicinal material was soaked by 10-fold 50% ethanol solution for 45min, and then extracted by heating reflux for 3 times with 1.5h each time. Conclusion： The method can effectively extract the ethanol dissolvable ingredients in 10 kinds of medicinal materials, which can be used as the ethanol extraction technology for Xiaoluo granules.