顶空气相色谱法测定小儿复方麻黄碱桔梗糖浆中薄荷脑的含量

Determination of Menthol in Xiao'er Fufang Mahuangjian Jiegeng Syrups by HS-GC

目的：建立顶空气相色谱法测定小儿复方麻黄碱桔梗糖浆中薄荷脑的含量。方法：采用Agilent DB-WAX毛细管柱（30 m×0.53 mm,1.0μm）,FID检测器;进样口温度为：200℃,柱温：150℃,检测器温度：250℃;载气（氮气）流量：2.0 ml·min-1;分流比：1∶1;顶空平衡温度：80℃,平衡时间：30 min,进样体积：1 ml。结果：在该色谱条件下,薄荷脑在2.09~20.92μg·ml-1的浓度范围内与峰面积呈良好的线性关系（r=0.999 1）;平均加样回收率为98.0%,RSD为1.8%（n=6）。结论：该方法简便、准确,重复性好,可用于测定小儿复方麻黄碱桔梗糖浆中薄荷脑的含量测定。

Objective： To establish an HS-GC method for the determination of menthol in Xiao＇er Fufang Mahuangjian Jiegeng syrups. Methods： An Agilent DB-WAX column（ 30 m × 0. 53 mm,1. 0 μm） with an FID detector was used; the inlet temperature was150℃,the detector temperature was 250℃; the flow rate of the carrier gas（ N2） was 2.0 ml·min- 1,the split ratio was 1∶1; the headspace equilibrium temperature was 80℃,the balance time was 30 min,and the sample volume was 1ml. Results： The calibration curve of menthol was within the range of 2. 09-20. 92 μg·ml- 1,which showed a good linear relationship（ r =0.999 1）. The average recovery of menthol was 98. 0%（ RSD = 1. 8%,n = 6）. Conclusion： The method is simple,accurate and reproducible,and can be used for the determination of menthol in Xiao＇er Fufang Mahuangjian Jiegeng syrups.