HPLC法同时测定氯甲醑中氯霉素和甲硝唑的含量

Simultaneous Determination of Chloromycetin and Metronidazole in Chlortalidone and Metronidazole Spirits by HPLC

目的：建立HPLC法同时测定氯甲醑中氯霉素及甲硝唑的含量。方法：采用HPLC法,色谱柱为Nov-pak C18（150mm×4.6 mm,5μm）,流动相为甲醇∶水（75∶25）,流速为1.0 ml·min-1,检测波长为297 nm,柱温：30℃,进样量20μl。结果：甲硝唑和氯霉素线性范围分别为5~80μg·ml-1（r=0.999 7）和50~800μg·ml-1（r=0.999 8）;平均回收率分别为100.83%（RSD=1.82%,n=6）和100.20%（RSD=0.55%,n=6）。结论：本法快速方便、精密度高、重复性和稳定性好,可用于同时测定氯甲醑中氯霉素和甲硝唑的含量。

Objective： To establish an HPLC method for the simultaneous determination of chloromycetin and metronidazole in chlortalidone and metronidazole spirits. Method： The HPLC method was performed on an NOV-pak C18（ 150 mm ×4.6 mm,5 μm）column and the mobile phase consisted of methanol and water（ 75∶ 25）. The flow rate was 1. 0 ml·ml- 1,the detection wavelength was 297 nm,the temperature of column was 30℃ and the sample size was 20 μl. Result： The calibration curve of metronidazole was linear within the range of 5-80μg·ml- 1（ r =0.999 7）,and the average recovery was100.83% with RSD of 1.82%（ n =6）. The calibration curve of chlortalidone was linear within the range of 50-800μg·ml- 1（ r =0.999 7）,and the average recovery was 100.2%with RSD of 0. 55%（ n = 6）. Conclusion： The method is simple,rapid,accurate and reproducible,and can be used in the quality control of chlortalidone and metronidazole spirits.