人C-kit阳性心脏干细胞离子通道特性

Functional ion channels in human c-kit+cardiac stem cells

目的研究不同人来源的人左心耳组织培养的c-kit+(CD117)心脏干细胞(c-kit+CSCs)的离子通道的表达情况,进一步了解心脏干细胞的电生理特性,为临床干细胞移植的安全性提供参考。方法通过分离筛选培养人c-kit+CSCs,运用全细胞膜片钳和定量反转录聚合酶连锁反应(qRT-PCR)技术,检测人c-kit+CSCs的通道电流和MaxiK基因、HNE-Na基因、CACNA1G基因、Kv4.2基因、CACNA1C基因的表达情况。结果在人c-kit+CSCs上,记录到了人电压依赖性Ca2+激活K+电流,检测到MaxiK基因表达相对较高,HNE-Na基因、CACNA1G基因表达尚可,Kv4.2基因通道的表达相对较低,未见CACNA1C基因的表达。结论人c-kit+CSCs表达多种离子通道的相关基因,能记录到人电压依赖性Ca2+激活K+电流,这与其基因的高表达是相一致的。

Objective To investigate functional ion channels in undifferentiated human c-kit （CDl17） cardiac stem cells （c-kit＋ CSCs）, learn more about cardiac stem cell electrophysiological characteristics for the purpose of clinical stem cell transplantation security. Methods Isolation of cultured human c-kit＋ CSCs, using whole-cell patch clamp and quan- titative reverse transcription polymerase chain reaction （qRT-PCR） technology to detect human c-kit＋ CSCs channel cur- rents and the expression of MaxiK gene, HNE-Na gene, CACNA1G gene, hKv4.2 gene, CACNA1C gene. Results In the human c-kit＋ CSCs, the whole-cell patch clamp technique to human voltage-dependent Ca activated K＋ current, qRT- PCR gene expression detected MaxiK relatively high, HNE-Na gene, CACNA1G gene expression is acceptable, hKv4. 2 gene expression in the channel is relatively low, no CACNA1C gene expression. Conclusion C-kit＋ CSCs express variety of ion channel genes, it can be recorded through the human voltage-dependent Ca2＋ activated K＋ current, which is consistent with gene＇s high expression.