长期高温环境对大鼠免疫系统影响的研究

Influence of long-term high-temperature environment on immune system of rats

目的通过观察长期高温环境下大鼠血清中IL-1(白介素1),IL-2(白介素2)及溶菌酶活力的变化,进一步探讨高温对免疫功能的影响。方法健康成年SD大鼠40只,体重(200±20)g,雌雄各半,随机分为高温组和对照组。人工建立(38±1)℃高温环境模型,每周连续处理7 d,每天处理2 h。经过80和150 d后,解剖各组大鼠,称脏器重量,并检测血清中细胞因子IL-1、IL-2含量及溶菌酶活力,评价大鼠免疫功能变化。结果高温热暴露80 d后,大鼠脾脏系数、肝脏系数分别为(0.202 1±0.020 3)%、(4.321 5±0.846 7)%,与对照组的差异有统计学意义(P<0.05)。血清中IL-1含量为(58.686 0±18.648 9)g/ml,与对照组的(24.934 0±8.550 2)g/ml相比,差异有统计学意义(P<0.01);IL-2含量[(46.624 0±114.348 1)g/ml]和溶菌酶活力[(139.132 0±23.375 2)mg/l],与对照组的差异均有统计学意义(P<0.05,P<0.01)。高温热暴露150 d后,大鼠脾脏系数为(0.173 7%±0.016 8%),与对照组的差异有统计学意义(P<0.05),其他脏器系数无明显变化(P>0.05);血清中IL-1(白介素1)、IL-2(白介素2)含量和溶菌酶活力与对照组的差异均无统计学意义(P>0.05)。结论长期高温暴露对大鼠的免疫功能有一定影响,同时长期高温刺激可在一定程度上提高大鼠免疫耐受能力。

[Objective]To observe the change of IL-1,IL-2 and lysozyme vitality in rats＇ serum under long-term high-temperature environment,explore the influence of hyperthermia on immune function. [Methods]A total of 40 healthy SD rats were selected,whose weight were（200 ± 20）g,half were male and hale were female. They were randomly divided into hyperthermia group and control group. High-temperature environment model [（38 ± 1） ℃]were built,the rats were under the model 2h per day,7 days per week. After 80 and 150 days,rats were dissected,organs were weighed,the contents of IL-1 and IL-2 and lysozyme vitality were detected,and the change of immune function was evaluated. [Results]After exposure to high-temperature environment 80 days,the liver and spleen coefficient of rats were（0. 202 1 ± 0. 020 3）% and（4. 321 5 ± 0. 846 7）%,compared with the control group,the difference was statistically significant（ P〈0. 05）. The content of IL-1 was（58. 686 0 ± 18. 648 9） g /ml,compared with the control group（24. 934 0 ± 8. 550 2） g /ml,the difference was statistically significant（P 0. 01）. The content of IL-2 and lysozyme vitality were（46. 624 0 ± 114. 348 1） g /ml and（139. 132 0 ± 23. 375 2） g /ml,compared with the control group,the difference was statistically significant（P〈0. 05,P 〈0. 01）. After exposure to high-temperature environment 150 days,the spleen coefficient of rats was（0. 173 7 ± 0. 016 8）%,compared with the control group,the difference was statistically significant（P 0.05）. The differences of other organ coefficient were not statistically significant（P〉0. 05）. The differences of IL-1 and IL-2 contents and lysozyme vitality between hyperthermia and control group were not statistically significant（ P〉0. 05）.[Conclusion]There is influence of long-term high-temperature environment on immune function of rats. Meanwhile rats＇ immune tolerance can be improved by long-term high-temperature stimulation.