铬离子对人成骨样细胞MG63增殖活性的影响及拮抗研究

Effects and antagonism of chromium ion on proliferation of human MG63 cell

目的研究金属铬离子对成骨细胞增殖活力的影响以及抗氧化剂N-乙酰半胱氨酸（N-acetyl-cysteine,NAC）对其拮抗效应。方法用不同浓度（0~20μM）的Cr6＋和Cr3＋诱导人成骨样细胞株MG63,采用四甲基偶氮唑盐法检测细胞增殖活力,并检测不同浓度（0~5 mM）NAC对金属铬离子细胞毒性的拮抗作用。结果在同一时间点,随着Cr6＋浓度增加,MG63细胞增殖活性显著降低,呈一定剂量-效应关系;在相同浓度组,72 h较24 h诱导细胞增殖活力降低（P〈0.05）;Cr3＋对MG63细胞增殖无明显影响（P〉0.05）;与10μM Cr6＋处理组相比,Cr6＋＋NAC组随着NAC浓度增加,细胞增殖活力显著增加（P〈0.05）;2 mM NAC＋10μM Cr6＋组、5 mM NAC＋10μM Cr6＋组在24 h和72 h检测点,与空白对照组细胞增殖活力差异无统计学意义（P〉0.05）。结论 Cr6＋对MG63细胞具有细胞毒性,随浓度增大;NAC可拮抗其细胞毒性,随浓度增强;尚不能表明Cr3＋对MG63有明显细胞毒性。

Objective The objective was to investigate the influence of different concentration and stage of chromium ion in MG63 cell＇ s viability as well as if antioxidant N-acetyl-cysteine reduced the cytotoxieity induced by chroraium ion. Methods MG63 cell lines were exposed in vitro for 24 h or 72 h to different concentration of Cr^6 ＋ and Cr^3＋ （5 μM, 10 μM, 20 μM） FI2 medium. Cr^6＋ and Cr^3＋ were obtained from their metal salts solution. Anti also, in order to assess the ability of antinxidant NAC to protect MG63 against chromium ion-induced cytotoxity, MG63 were preincubated with （1 μM, 2 μM, 5 μM） NAC for 30 rain, and then euhured with 10 μM Cr^6＋. F12 medium for further 24 h or 72 h. Cellular survival rates were evaluated by the MTT assay. Results A timeand conc＇entrationdependent increased cvtoloxicitv in MG63 cell lines were fonnd when exposure to Cr^6＋. Treatment of MG63 cells with NAC aftorded dose-dependent rcdt, ction to the cytotoxicity. On the contrary, Cr^3＋ has no significant influence in MG63 cell＇ s survival rate in our study. Conclusion Cr^6＋ has significant eytotoxicity in MG63 cells. Antioxidant N-acetyl-cysteine can play a critical role against Cr^6＋ -induced cytotoxicity. The concentration of Cr^3 ＋ in this study （5 μM -20 μM） have no significantly cytotoxicity in MG63 cell lines.