胰高血糖素样肽-2对急性胰腺炎小鼠肠黏膜免疫屏障的影响

Effect of glucagon-like peptide-2 on intestinal mucosal immune barrier in mice with acute pancreatitis

目的：探讨胰高血糖素样肽-2（GLP-2）对重症急性胰腺炎（SAP）小鼠肠黏膜免疫屏障的影响。方法健康雌性BALB/C小鼠60只,按数字表法随机分成3组：正常对照组（ NC组）、SAP组和GLP-2治疗组（ GLP组）,每组20只。 SAP组、GLP-2组采用腹腔分次注射大剂量L-精氨酸方法制备SAP模型。观察SAP小鼠血和腹水淀粉酶及肠黏膜CD3＋、CD4＋、CD8＋淋巴细胞和IgA λ链的改变,应用GLP-2治疗后观察上述指标的改变。结果 SAP 组小鼠血和腹水淀粉酶值分别为（1260.93±58.99）U/L和（389.33±30.31） U/L,均高于 NC组和 GLP-2组,差异均有统计学意义（P值均〈0.01）。免疫组化法检测SAP组小鼠肠黏膜CD3＋、CD4＋、CD8＋淋巴细胞和IgA λ链的表达分别为（19.57±6.56）个/HP、（5.49±2.97）个/HP、（2.96±1.22）个/HP、（6.20±3.22）个/HP,低于NC组和GLP-2组,差异均有统计学意义（P值均〈0.05）；但GLP-2治疗组与NC组比较,CD4＋淋巴细胞和IgAλ链的表达差异均无统计学意义（P值均〉0.05）。结论 GLP-2能够提高SAP小鼠肠黏膜CD3＋、CD4＋、CD8＋淋巴细胞和IgA λ链的表达,对肠黏膜免疫屏障具有一定的保护作用。

Objective To investigate the influences of glucagon-like peptide-2（GLP-2）on intestinal mucosal immune barrier in mice with sever acute pancreatitis（ SAP） . Methods All healthy female BALB/C mice were divided into normal control, SAP and GLP-2 group by random allocation. SAP mice model was established by intraperitoneal injections with large dose L-Arg. The changes of CD3 ＋, CD4 ＋, CD8 ＋lymphocyte and IgA λchain of intestinal mucous membrane and amylase of blood and ascites were observed. The same method was used in GLP-2 group to observe the changes of the above indices. Results The blood and ascites amylase value in SAP groups were （ 1 260. 93 ± 58. 99 ） U/L and （ 389. 33 ± 30. 31 ） U/L respectively, were significantly higher than those in NC group and GLP-2 treatment group （ all P values〈0. 01）. Expression of SAP in intestinal mucosa of mice CD3 ＋, CD4 ＋, CD8 ＋ lymphocytes and IgA λchain were （ 19. 57 ± 6. 56 ）/HP, （ 5. 49 ± 2. 97 ）/HP, （ 2. 96 ± 1. 22 ）/HP and （ 6. 20 ± 3. 22 ）/HP respectively, were obviously higher than those of the NC group and the SAP group（all P values〈0. 05）. Compared with the NC group, expression of mice CD4 ＋ lymphocytes and IgAλ chain of GLP-2 treatment group had no statistical significance （all P values 〉 0. 05）. Conclusions GLP-2 can increase the expression of CD3 ＋, CD4 ＋, CD8 ＋ lymphocytes and IgAλchain in intestinal mucosa of SAP mice, and has the protective effect on intestinal immune barrier.