慢性丙型肝炎患者血清prohibitin表达水平的检测

The expression of prohibitin in serum of patients with chronic HCV infection

目的检测抗增殖蛋白(prohibitin,PHB)在慢性丙型肝炎患者血清中的表达水平,为探讨PHB在丙型肝炎病毒(HCV)致病机制中的作用提供临床资料。方法应用双抗体夹心法ELISA定量检测63例慢性丙型肝炎患者及30例正常体检者血清PHB浓度,同时检测丙型肝炎患者血清病毒滴度及肝功能指标。结果丙型肝炎患者及正常人血清PHB浓度分别为31.88 ng/L及76.94 ng/L,丙肝患者血清PHB含量明显低于正常人(U=344.00,P=0.000),丙肝患者肝功能指标天冬氨酸转氨酶(AST)、直接胆红素(DBIL)与PHB浓度呈负相关(r=-0.484,P=0.012;r=-0.446,P=0.022)。结论慢性丙型肝炎患者血清PHB含量低于正常人,并与肝损伤的严重程度呈负相关,肝损伤越重,PHB含量越低。

Objective To detect the expression of prohibitin in serum of patients with chronic HCV infection,to provide clinical data for the role of prohibitin in pathogenic mechanism of hepatitis C virus.Methods The concentration of prohibitin in serum of 63 chronic HCV infection patents and 30 healthy controls were quantitately examined by ELISA.At the same time,HCV RNA titers and liver function index were also detected.Results The concentrations of prohibitin in serum of chronic HCV infection patients and healthy controls were 31.88 ng/L and 76.94 ng/L,respectively.The concentration of prohibitin in HCV patients was significant lower than that in healthy controls （U =344.00,P =0.000）.The expression of prohibitin in patients serum had negative correlation with acpart aminotranferase （AST） （r =-0.484,P =0.012） and direct bilitubin （DBIL） （r =-0.446,P =0.022）.Conclusion Prohibitin was down-expressed in HCV patients.