槲皮素联合顺铂对人骨肉瘤MG-63细胞增殖及凋亡的影响

Effect of quercetin combined with cisplatin on proliferation and apoptosis of human osteosarcoma cell line MG-63

目的研究槲皮素联合顺铂对人骨肉瘤MG-63细胞增殖及凋亡的影响,并初步探讨其作用机制。方法采用不同浓度槲皮素与顺铂单独及联合作用MG-63细胞;倒置相差显微镜观察细胞形态变化;CCK-8法检测细胞增殖抑制率,并通过Chou-Talaly联合指数法分析两药之间相互作用;流式细胞仪检测细胞凋亡率;RT-PCR及Western blot检测Bcl-2、caspase-3等凋亡相关分子在基因及蛋白水平的表达变化。结果槲皮素及顺铂均能抑制MG-63细胞增殖并诱导其凋亡,两药联合具有协同效应,并可下调Bcl-2及上调caspase-3基因及蛋白的表达。结论槲皮素联合顺铂能协同抑制MG-63细胞增殖并诱导其凋亡,其作用机制可能与下调Bcl-2及上调caspase-3等凋亡相关分子的表达有关。

Aim To investigate the effect and mecha-nism of quercetin combined with cisplatin on prolifera-tion and apoptosis of human osteosarcoma cell line MG-63 . Methods MG-63 cells were treated with quercetin alone or combined with cisplatin. Cellular morphologic changes were observed under inverted phase contrast microscope. The effects of proliferation inhibition were assayed by CCK-8 method. The combination effect was judged through Chou-Talaly analysis. The apoptosis ra-tios of cells were analyzed by flow cytometry. The gene expression of Bcl-2 and caspase-3 was detected by RT-PCR assay. The protein expression of Bcl-2 and caspase-3 was measured by Western blot assay. Re-sults Quercetin alone or combined with cisplatin could inhibit the proliferation, but induce the apoptosis of MG-63 cells. Combination of quercetin and cisplatin revealed a synergistic effect on cell proliferation and apoptosis as it reduced the expression of Bcl-2 but en-hanced that of caspase-3 at both gene and protein lev-els. Conclusion Synergistic effect of quercetin com-bined with cisplatin on cell proliferation and apoptosis of MG-63 cells is possibly due to reduction of Bcl-2 and enhancement of caspase-3 expression.