脉络宁注射液对脂多糖与角叉菜胶诱导“热毒血瘀证”模型大鼠的NF-κB和TNF-α影响

Effect of Mailuoning Injection on NF- κB and TNF- α of Rat Models with Noxious Heat Stasis Syndrome Induced by Lipopolysaccharide Combined with Carrageenan

目的:研究脉络宁注射液对脂多糖与角叉菜胶诱导"热毒血瘀证"模型大鼠的核转录因子κB(NF-κB)和肿瘤坏死因子α(TNF-α)的作用。方法:50只SPF级SD雄性大鼠随机分为空白对照组,模型对照组和脉络宁高、中、低剂量组(30mg/kg,15mg/kg,3.75mg/kg),每组10只,腹腔注射给药20天,每天1次。给药到18天,除空白对照组外,其余各组大鼠腹腔注射Ca(50mg/kg)16h后,再尾静脉注射LPS(5μg/kg),24h后大鼠尾部出现瘀血现象为模型建立成功。给药20天后,从腹主动脉取血和取脾。检测血液的红细胞比积和活化部分凝血活酶时间,光镜下观察脾脏的病理变化,免疫组化法检测各组大鼠脾脏的核转录因子κB(NF-κB)和肿瘤坏死因子α(TNF-α)。结果:与模型对照组比较,脉络宁高剂量组的红细胞比积,NF-κB和TNF-α显著减少(P<0.05),活化部分凝血活酶时间显著缩短(P<0.05),脾脏的脏器系数显著增加(P<0.05)。结论:脉络宁注射液对脂多糖与角叉菜胶诱导"热毒血瘀证"模型大鼠有一定的活血抗炎作用。

Objective： To study the effect ot Mattuonmg lnjecuon（ TNF）on NF -κB, els with noxious heat stasis syndrome induced by lipopolysaceharide（LPS） combined with carrageenan （Ca）. Methods： Fifty SPF SD male rats were randomly divided into a blank control group, a model control group and high, middle and low dose of Mailuoning groups （30mg/kg, 15mg/kg,3.75mg/kg）. Rats in the model group were pretreated with Ca i.p. at the doses of 50mg/kg, then treated with LPS i.v. at the doses of 5 μg/kg six- teen hours later. After 24 hours, rat tails had the phenomenon of congestion which was considered as success-ful rat models. Blank control and model control groups were injected with the same capacity of NS. Low, mid- dle and high dose of Mailuoning groups were given Mailuoning by injecting intraperitoneally （3.75mg/kg, 15mg/kg,30mg/kg） , for 20 consecutive days, once a day. After the first 18 days administration, rats of mod- el control group and each dose of Mailuoning groups were being models. Their blood was collected from the ab- dominal aorta and removed spleen. We detected red blood cells and measured the activated clotting time and observed the histopathology of spleen by light microscopy. Results： Red blood cells and the expressions of NF - κB and TNF - α correspondingly were reduced （ P 〈 O. 05 ） ; the activated clotting time was shortened （P 〈 O. 05 ）, and the weight of spleen increased （ P 〈 0.05 ）. There were no obvious inflammation and con- gestion symptoms in the high dose of Mailuoning group compared with those of model group. Conclusion ： Mai- luoning injection has an anti - inflammatory effect on the rat models with noxious heat stasis syndrome induced by lipopolysaccharide combined with carrageenan.