舒心稳斑颗粒对兔AS斑块稳定性及MMP-9、I型胶原蛋白表达的影响

Effects of Shuxin Wenban Granule on Stability of Rabbits' AS Plaque and Expressions of MMP-9 and COL-I

目的:观察舒心稳斑颗粒对实验兔AS斑块稳定性及MMP-9、I型胶原蛋白表达的影响。方法:运用高脂高胆固醇饲料喂养12周制备日本大耳白兔动脉粥样硬化模型,用舒心稳斑颗粒灌胃给药治疗4周,采用双抗体夹心酶联免疫吸附法检测血清中I型胶原蛋白的含量;采用免疫组化法分析I型胶原蛋白、MMP-9在主动脉粥样斑块中的表达;同时观察主动脉粥样硬化斑块的病理学改变。结果:与模型组比较,舒心稳斑颗粒能升高血清中Ⅰ型胶原蛋白的含量,差异具有显著统计学意义(P<0.01);同时能降低斑块中MMP-9的表达水平,升高Ⅰ型胶原蛋白在斑块中的表达水平。病理学检查结果显示,采用舒心稳斑颗粒干预后,主动脉粥样硬化斑块的病理学形态、结构均发生改变,斑块结构更加趋于稳定。结论:舒心稳斑颗粒可能通过抑制斑块内炎症反应,调节动脉粥样硬化斑块纤维帽中的胶原纤维降解与合成失衡状态,进而对AS斑块起稳定作用。

Objective：To observe the effects of Shuxin wenban granule on the stability of AS plaque and the expressions of MMP-9 and COL-l in rabbits.Methods： Using the high cholesterol diet to induce AS rabbit models for 12 weeks, then, giving Shuxin wenban granule for 4 weeks, finally,detecting the serum levels of COL-I in the rabbits by double antibody sandwich enzyme -linked immunosorbent method and the surface density of MMP-9 and COL-I in atherosclerotic plaques by immunohistochemical staining .At the same time , observing the pathological changes of aorta in atherosclerotic rabbits .Results：To compared with model group , Shuxin wen-ban granule could increase the serum levels of COL -I,the difference was statistically significant （P〈0.01）;similarly, Shuxin wenban granule could significantly decrease the surface density of MMP -9 and increase the surface density of COL-I in atherosclerotic plaques,the difference was statistically significant （P〈0.01）.Pa-thology showed that Shuxin wenban granule changed the pathological morphology , structure of atherosclerotic plaque , caused the plaque to stabler .Conclusion： Shuxin wenban granules could stabilize atherosclerotic plaques by inhibiting the inflammatory response of atherosclerotic plaque and affecting the imbalance of collagen fibers’ degradation and synthesis in atherosclerotic plaque fibrous cap .