灰毡毛忍冬新品种‘渝蕾一号’的组织培养研究

Tissue Cultivation of Lonicera macranthoides ‘Yulei1'

目的：建立灰毡毛忍冬新品种‘渝蕾一号’Lonicera macranthoides Hand.-Mazz.‘Yulei1’组培快繁体系,为实现其工厂化育苗提供理论依据。方法：以‘渝蕾一号’幼嫩茎段和叶片为外植体,以MS为基本培养基,在组织培养的不同阶段添加不同浓度的NAA、6-BA、TDZ、IBA等植物生长调节物质,对愈伤组织的诱导、增殖及分化,茎段腋芽诱导、增殖及生根炼苗进行系统研究。结果：外植体的最佳灭菌时间：茎段为2~3 min,叶片为1~2 min;愈伤组织诱导最佳培养基为MS＋2,4-D 1.0 mg/L＋6-BA 0.1 mg/L;腋芽诱导最佳培养基为MS＋6-BA 1.0 mg/L＋NAA0.5 mg/L;愈伤组织增殖最佳培养基为MS＋TDZ（0.2~1.0）mg/L＋NAA（0.02~0.05）mg/L;愈伤组织分化最佳培养基为MS＋6-BA（0.5~1.0）mg/L＋NAA（0.02~0.05）mg/L;芽增殖最佳培养基为MS＋6-BA 1.5 mg/L＋NAA0.5 mg/L;最佳生根培养为1/2MS＋IBA 1.0 mg/L＋NAA 0.2 mg/L。试管苗在珍珠岩和腐殖质（1∶1）基质中炼苗,成活率达75%以上。结论：筛选出了外植体适宜的消毒方法及愈伤组织诱导、增殖、分化及芽增殖、生根的适宜培养基,建立了‘渝蕾一号’组培快繁体系。

Objective： To establish the rapid tissue propagation system of Lonicera macranthoides＇Yulei1＇,in order to provide theoretical basis for industrialized seed cultivation.Methods： Young stem and leaves of Lonicera macranthoides＇Yulei1＇were used as explants,with MS as basic media,and added with different concentrations of plant growth regulators such as NAA,6-BA,TDZ and IBA in different stages of tissue culture,in order to conduct a systematic study on callus induction,proliferation and differentiation,and stem axillary bud induction,proliferation,rooting and seedlings.Results： Best sterilization time of explant was： stem for 2 ~ 3 min,leaves for 1 ~2 min.The optimal medium for callus induction was MS ＋ 2,4-D 1.0 mg /L ＋ 6-BA 0.1 mg /L.The optimal medium for axillary bud induction,callus proliferation,callus differentiation,bud proliferation and rooting was MS ＋ 6-BA 1.0 mg /L ＋ NAA 0.5 mg /L,MS ＋ TDZ（ 0.2 ~1.0） mg/L ＋NAA（ 0.02 ~0.05） mg/L,MS ＋6-BA（ 0.5 ~1.0） mg/L ＋ NAA（ 0.02 ~0.05） mg/L,MS ＋6-BA 1.5 mg/L ＋NAA 0.5 mg /L and 1 /2MS ＋ IBA 1.0 mg /L ＋ NAA 0.2 mg /L,respectively.Tube seedlings was refined in matrix include pearlite and humus（ 1∶ 1）,with the survival rate of more than 75%.Conclusion： The disinfection method suitable for explants and the medium combination suitable for callus induction,proliferation,differentiation as well as bud proliferation and rooting are screened out to establish the rapid cultivation system for Lonicera macranthoides＇Yulei1＇.