灯盏花素脂质体冻干粉的制备及性质研究

Study on Preparation of Breviscapine Liposomes and Chemicophysical Properties

目的:制备灯盏花素脂质体冻干粉,并对其理化性质进行研究,以考察其稳定性与模拟生物膜的结合情况。方法:采用薄膜分散-冻干法制备灯盏花素脂质体冻干粉,用电导法测定灯盏花素脂质体的相转变温度,并进行聚集动力学研究。用水-正辛醇扩散模型试验来模拟体内生物膜,通过测定药物及脂质体从水向正辛醇扩散的速率,考察其在两相间的动态分配行为。结果:脂质体混悬液的相转化温度为63℃,聚集活化能为14.66 kJ/mol。通过对药物在水-正辛醇体系中的动态分布研究表明,灯盏花素脂质体在该模拟的生物膜表面药量大于普通输液。结论:灯盏花素脂质体稳定性较好,有利于改善疗效。

Objective： To prepare breviscapine pro-liposomes and evaluate its properties and stability,as well as its interaction with the mimic-membrane.Methods： Breviscapine liposomes were prepared by thin film-lyophilization method.Phase inversion temperature was measured by electrical conductance method and coalescence kinetics was studied.Water /n-octanol trans-membrane diffusion model was designed to study the dynamic distribution behavior between two phases,through the determination of diffusion rate of breviscapine and liposomes.Results： The phase inversion temperature was 63 ℃,the activity energy for coalescence was 14.66 kJ /mol.The results suggested that breviscapine from liposomes staying on the interface were found more than the breviscapine infusion.Conclusion： Breviscapine liposomes prepared with thin film-lyophilization method have good physicochemical properties and stability,which is beneficial to treatment.