不同配比辛芍组方对PC12细胞氧糖剥夺损伤的保护作用

Protective Effects of Different Proportions of Herba erigerontis and Radix paeoniae rubra on PC12 Cells against Oxygen Glucose Deprivation Induced Injury

目的:观察不同配比辛芍组方对PC12细胞氧糖剥夺损伤的保护作用,探讨组方的最佳配比。方法:采用连二亚硫酸钠(Na2S2O4)建立PC12细胞氧糖剥夺损伤(oxygen glucose deprivation,OGD)模型,将细胞分为空白组、模型组、阳性组(尼莫地平)、辛芍药物不同配比组(灯盏细辛与赤芍质量比分别为5∶0、4∶1、3∶2、2∶3、1∶4、0∶5),检测细胞存活率、乳酸脱氢酶(LDH)释放量、超氧化物歧化酶(SOD)活性、丙二醛(MDA)和一氧化氮(NO)含量。结果:与正常组比较,模型组的细胞存活率和SOD活性显著降低(P<0.01),细胞LDH释放量、MDA和NO水平显著升高(P<0.01);与模型组比较,辛芍2∶3配比组显著提升细胞存活率(P<0.05),同时辛芍3∶2和2∶3配比组均显著降低细胞LDH释放量以及MDA和NO水平(P<0.05),提高SOD活性(P<0.05)。结论:辛芍2∶3配比对PC12细胞氧糖剥夺损伤的保护作用较好。

Objective： To investigate the optimum proportion for the formula of Herba erigerontis and Radix paeoniae rubra through comparative investigations of the protective effects on PC12 cell against oxygen glucose deprivation （OGD）-induced injury. Methods： The OGD-induced injury model was es- tablished by adopting Na2S204. Cells were divided into control group, OGD group, positive group （ni- modipine）, formula group （ proportion as 5： 0,4： 1,3 ： 2,2： 3,1 ： 4,0： 5 ）. It was aimed to detect cell survival, lactate dehydrogenase （LDH） release, superoxide dismutase （SOD） activity, malondialde- hyde （MDA） and Nitric Oxide （NO）. Results： Compared with control group, the OGD model de- creased the survival rate and the activity of SOD （P 〈0.01 ）, and increased the LDH release, the lev- el of MDA and NO（P 〈0.01 ） ; Compared with OGD group, the 2：3 formula group significantly in- creased the survival rate （ P 〈 0.05 ）, while the 3 ： 2 and 2 ： 3 formula groups were significantly de- creased the LDH release, the level of MDA and NO （ P 〈 0.05 ）, increased SOD activity （ P 〈 0.05 ）. Conclusions： The 2：3 formula of Herba erigerontis and Radix paeoniae rubra has significant protective effects on the PC12 cells injured by OGD.