补阳还五汤对心肌梗死大鼠心肌细胞凋亡及Hsp20表达的影响

Influence of Buyanghuanwu Decoction on Myocardial Cell Apoptosis and Heat Shock Protein 20 Expression in Myocardial Infarction Rats

目的:探讨补阳还五汤对心肌梗死大鼠左室非梗死区心肌细胞凋亡及热休克蛋白20(Hsp20)表达的影响。方法:采用SPF级雄性Wistar大鼠,将大鼠随机分成模型组、补阳还五汤组和假手术组;模型组和补阳还五汤组予结扎左冠状动脉主干造成急性心肌梗死,假手术组行手术操作,但不结扎冠状动动脉;采用TUNEL染色检测3组大鼠心肌细胞凋亡情况,Western-blot法检测3组大鼠心肌细胞中Hsp20和磷酸化-Hsp 20(p-Hsp20)的表达,qPCR法检测Hsp20 mRNA的表达。结果:与假手术组比较,补阳还五汤组的凋亡细胞增多,凋亡指数增加,两组比较差异有统计学意义(P<0.01);与模型组比较,补阳还五汤组细胞凋亡明显减少,凋亡指数降低,两组比较差异具有统计学意义(P<0.01);补阳还五汤组心肌细胞中Hsp20及p-Hsp 20表达较模型组增高,较假手术组降低,差异具有统计学意义(P<0.01);补阳还五汤组心肌细胞中Hsp20 mRNA的表达较模型组升高,较假手术组降低,差异具有统计学意义(P<0.01)。结论:补阳还五汤可能通过促进Hsp20的表达,从而起到抑制心肌细胞凋亡的作用。

Objective： To investigate the effects of Buyanghuanwu decoction on myocardial cell apop- tosis and heat shock protein 20 （Hsp20） expression at the non-infarction regions of left ventricle of my- ocardial infarction rats. Methods： One hundred and twenty male Wistar SPF rats were used. Acute myocardial infarction model was established by ligating the left main coronary artery. Rats were divided into model group （group M） , Buyanghuanwu decoction group （group B） and sham group （group S）. TUNEL staining was adopted to detect myocardial cell apoptosis and Westren-blot was performed to de- tect Hsp20 and phosphorylation-Hsp 20 （ p-Hsp 20） expression in myocardial cells of all three groups. Hsp20 mRNA expression was detected by using qPCR. Results： Comparing with group S, both apop- totic cell number and apoptotic index of group B increased, and the differences between the two groups were statistically significant （P 〈0.01 ） ; comparing with group M, apoptotic cell number reduced and apoptotic index decreased in group B, and the differences were statistically significant （ P 〈 0.01 ） ; myocardial cell Hsp 20 and p-Hsp 20 expressions of group B were significantly higher than those of group M, and obviously lower than those of group S （ P 〈 0.01 ） ; The myocardial cell Hsp 20 mRNA expression in group B was more than in group M, and lowered more than in group S. The differences were statistically significant （ P 〈 0.01 ）. Conclusions： Buyanghuanwu decoction might promote the expression of Hsp 20 to inhibit myocardial cell apoptosis in the non-infarction regions of left ventricle in rats with acute myocardial infarction.