UFLC-UV法同时测定黔产丹参中4个水溶性成分的含量

UFLC-UV Simultaneous Determination of Four Water-soluble Compounds in Salvia miltiorrhiza from Guizhou

目的：建立超快速液相色谱（UFLC）的方法同时测定丹参成分丹参素、迷迭香酸、丹酚酸B、丹酚酸A.方法：采用Diamonsil C18色谱柱（4.6 mm×250 mm,5μm）,以乙腈-0.05％磷酸为流动相进行梯度洗脱,流速1 mL/min,检测波长220 nm,柱温40℃,检测贵州4个不同产地的8批丹参成分丹参素、迷迭香酸、丹酚酸B、丹酚酸A.结果：4种被测成分的线性关系良好（r ＞0.999）,精密度RSD 0.49％ ～ 1.67％,重复性RSD1.27％ ～2.17％,稳定性RSD 1.53％～2.85％,平均加样回收率在96.80％～103.08％,RSD 1.88％ ～2.79％ （n=6）,8批黔产丹参药材中丹参素、迷迭香酸、丹酚酸B、丹酚酸A的平均含量分别为2.061、3.559、44.223及1.426 mg/g.结论：UFLC法用于检测丹参药材中丹参素、迷迭香酸、丹酚酸B、丹酚酸A,具有简便、快速、准确的优点,能有效控制丹参药材的质量.

Objective： To establish a UFLC-UV method for determination of four water-soluble com- pounds in Salvia miltiorrhiza of Guizhou. Methods： Chromatogaphic analysis was performed on a Dia- monsil Cls column （4.6 mm ×250 mm,5 μm） with acetonitrile -0.05% phosphoric acid as mobile phase in gradient elution mode. The flow rate was 1 mL/min with the detection wavelength of 220 nm. The column temperature was set at 40 ℃. 8 samples of 4 different producing areas from Guizhou were analyzed. Result： All the four water-soluble compounds showed good linearity （r 〉 0. 999 ） in the range of tests concentrations. The RSD of the precision, reproducibility and stability tests were 0.49% - 1.67%, 1.27% - 2.17%, 1.53% - 2.85% respectively ; the average recoveries of the method were in the range of 96.80% - 103.08%, RSD 1.88% - 2.79% （n = 6）. The average contents of four wa- ter-soluble compounds in Salvia miltiorrhiza of Guizhou were 2. 061,3. 559, 44. 223, 1. 426 mg/g re- spectively. Conclusions： The method is simple, rapid and accurate, and can be applied for the quali- ty control of Salvia miltiorrhiza from Guizhou.