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鸭ApoVLDL-Ⅱ基因第1内含子多态性及其遗传效应

Polymorphism and its genetic effects of duck ApoVLDL-Ⅱ gene in intron 1
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摘要 以褐色菜鸭为材料,对其ApoVLDL-Ⅱ基因第1内含子进行PCR-SSCP检测,分析其多态性及遗传效应.结果表明,在ApoVLDL-Ⅱ基因第1内含子检测到多态性,存在BB、Bb和bb 3种基因型,序列比对发现存在T374C、C379T和T390G 3个单核苷酸多态位点,该位点属中度多态,并处于Hardy-Weinberg平衡状态(P>0.05).关联分析显示,bb基因型个体的蛋重极显著高于BB型(P<0.01),bb基因型个体的蛋白重显著高于BB型(P<0.05).推测该片段的多态位点可能对褐色菜鸭蛋重和蛋白重有一定的影响. Brown Tsaiya ducks were adopted to analyze the diversity of ApoVLDL-Ⅱ gene intron 1 and its genetic effects using SSCP.The results showed that 3 genotypes （BB,Bb,bb） were identified in ApoVLDL-Ⅱ gene intron 1 and 3 single nucleotide polymorphism loci （T374C,C379T,T390G） were detected.These three polymorphism sites belong to medium genetic heterozygosis and achieved in Hardy-Weinberg equilibrium （P ＞ 0.05）.The individuals of genotype bb in egg weight was significantly heavier than that of genotype BB （P＜0.01）.And the genotype bb in egg-white weight was heavier than that of genotype BB （P ＜0.05）.Our results indicated that the polymorphisms of ApoVLDL-Ⅱ gene intron 1 might be related with egg weight and egg-white weight.
作者 林秋娟 任播扬 何桂娟 黄守婷 王景伟 肖天放
机构地区 福建农林大学动物科学学院 福建省畜牧总站
出处 《福建农林大学学报（自然科学版）》 CSCD 北大核心 2014年第4期403-407,共5页 Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金 福建省重大科技专项(2006NZ003-3) 福建省高等学校科技项目(JA09082)
关键词 ApoVLDL-Ⅱ基因 PCR-SSCP 多态性 褐色菜鸭 ApoVLDL-Ⅱ gene PCR-SSCP polymorphism Brown Tsaiya duck
分类号 S813.1 [农业科学—畜牧学]
  • 相关文献

参考文献16

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二级参考文献25

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共引文献5

福建农林大学学报（自然科学版）
2014年 第4期

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