姜黄素对小胶质细胞的活化的影响

The Effect of Curcumin on Microglial Activation

目的:研究姜黄素对脂多糖引起的小胶质细胞活化状态的影响,并探讨TOLR4受体在其中的作用。方法:采用体外培养N9小鼠小胶质细胞系,脂多糖作为刺激,激活小胶质细胞。采用ELISA法检测小胶质细胞培养基中TNF-α、IL-1β和IL-6的含量;相差显微镜观测细胞形态;免疫细胞化学和western blot观测小胶质细胞TOLR4受体表达情况。结果:与对照组相比,暴露于100ng/ml脂多糖24 h的小胶质细胞促炎症因子TNF-α、IL1β和IL-6释放量明显增加(P<0.05),姜黄素浓度为10μM时,可显著减少小胶质细胞释放TNF-α、IL-1β和IL-6(P<0.05),此外,姜黄素还可改善小胶质细胞形态,并降低暴露于脂多糖中的小胶质细胞TOLR4受体的表达。结论:姜黄素可能通过抑制TOLR4表达,减轻了脂多糖对小胶质细胞的激活。

Objective： To investigate the effect of curcumin （Cur） on lipopolysaccharide （LPS）-induced microglial actwatlon and the role of Toller Receptor 4 （TOLR4） in the effect. Methods： N9 microglia cell line exposed to LPS for 24 h was used to mimic the model of microglial inflammation. ELISA was used to assess the concentrations of tumor necrosis factor ct （TNF-a）, interleukin （IL）-1β and IL-6. Phase contrast microscope was used to record the morphology of microglial cells exposed to LPS. Immunocytochemistry and western blot were used to measure the expression of TOLR4. Results： Compared with that in the control, LPS of 100 ng/ml increased the concentrations of TNF-α, IL-1β and IL-6 in the supematant （P〈0.05）, while curcumin of 10 μM decreased the levels of TNF-α, IL-1β and IL-6 markedly （P〈0.05）. In addition, curcumin ameliorated the morphology and decreased the expression of TOLR4 in microglial cells exposed to LPS. Conclusion： Curcumin attenuated microglial activation induced by LPS, and the inhibition of TOLR4 may be associated with the effect.