NF-κB在哮喘病患儿支气管肺泡灌洗液中的表达及意义(英文)

A New Regulation Pathway in Asthma: NF-kB Activates the Expression of IL-17

目的:探讨NF-κB、IL-17在儿童支气管哮喘中的作用及机制。本文通过观察哮喘急性发作儿童支气管肺泡灌洗液(BALF)NF-κB、IL-17的水平变化,探讨其在哮喘急性发作儿童气道炎症中的作用。方法:我院2012年12月-2013年2月期间行纤维支气管镜检查患儿共80例,包括哮喘急性发作组(哮喘组,n=40)、非喘息组(肺炎组,n=20)及对照组(n=20),收集所有病例的BALF,进行细胞学分类,RT-PCR法测定BALF中细胞中NF-κB蛋白(P65mRNA)、IL-17mRNA的表达;Western法检测P65蛋白、IL-17蛋白表达。结果:与对照组比较,哮喘组和肺炎组患儿的P65mRNA、IL-17mRNA水平均明显增高;P65蛋白、IL-17蛋白水平均明显增高(均P<0.05);哮喘组患儿的P65mRNA、IL-17mRNA水平、P65蛋白、IL-17蛋白水平较肺炎组高(P<0.05)。结论:NF-κB、IL-17在哮喘儿童气道炎症中发挥重要作用,NF-κB通过调控IL-17来实现促进哮喘病情进展。

Objective： To investigate the role of NF-κB and IL-17 on the etiology of asthma and to explore the potential relation- ships between them. Methods： Bronchoalveolar lavage fluid （BALF） was obtained by fiberoptic bronchoscope setting into the trachea of asthmatic patients （n=40）, healthy individuals （n=20） and non-asthma patients（n=20）. The expressions of NF-κB （P65） mRNA and IL-17 mRNA were qualified through the RT-PCR and Western blotting. Results： The distribution of expression of P65 mRNA was significantly different between groupl-asthma group （acute attack）, group 2-non-asthma group and healthy controls. The relative capacity of P65 and IL-17 in asthma group was obviously higher than those of other groups. Conclusions： It is indicated that the IL-17 and NF-KB might be involved in the etiology of asthma, especially for the acute one. Our study also suggested a potential cause-effect association between IL-17 and NF-κB, which is the first investigation of the relationship between the two factors.