miR-29a靶基因预测及其相关生物信息学分析

miR-29a Target Genes Prediction and Related Bioinformatics Analysis

目的:通过对miR-29a进行靶基因预测及相关生物信息学分析,为miR-29a靶基因的实验验证提供数据支持,以期为深入研究miR-29a的生物学功能和调控机制提供理论指导。方法:利用PubMed检索miR-29a相关文章,通过miRBase在线工具分析miR-29a序列。应用TargetScan及miRNAda两种计算方法预测miR-29a靶基因并取其交集作为分析的基因集合,分别进行基因本体(gene ontology,GO)中的分子功能和生物学过程以及KEGG(Kyoto Encyclopedia of Genes and Genomes)生物通路富集分析。结果:(1)miR-29a序列在多物种间具有高度保守性。(2)两种方法预测miR-29a靶基因交集共191个。(3)miR-29a靶基因GO分子功能集中于转录因子活性、DNA结合和钙离子结合等(P<0.05);miR-29a靶基因GO生物学过程集中于调控转录、细胞粘附、细胞增殖与凋亡等(P<0.05);KEGG生物通路主要富集于PI3K-AKT信号通路、JAK-STAT信号通路、T细胞受体信号通路和胰岛素信号通路等信号转导通路,以及肺小细胞癌和子宫内膜癌等疾病通路(P<0.05)。结论:miR-29a可能通过参与多个靶基因信号通路的调控,在机体的多种生理病理过程中发挥重要作用,是一个颇有研究价值的生物学靶标。

Objective： Through the miR-29a target genes prediction and related bioinformatics analysis, the data provide experimental validation and theoretical guidance for further investigation of miR-29a related biological function and regulatory mechanisms. Methods： The studies of miR-29a were reviewed by Pubmed, and the sequence of miR-29a was obtained from miRBase. Target Scan and miRNAda were used to predict target genes of miR-29a, and the intersection of the two results was performed by Gene Ontology（GO） analysis and Kyoto Encyclopedia of Genes and Genomes（KEGG） pathway analysis. Results： （1） MiR-29a sequences were highly conserved in various species. （2） There were 191 predicted target genes in the gene set. （3） GO analyses ofmiR-29a target genes were enriched in transcription factor activity, DNA binding and calcium ion binding （GO molecular function, P〈0.05）, and enriched in regulation of transcription process, cell adhesion, cell proliferation and apoptosis （GO biology process, P〈0.05）; In KEGG pathway, the gene set mainly located in PI3K-AKT signaling pathway, JAK-STAT signaling pathway, T cell receptor signaling pathway and insulin signaling pathway （signal transduction pathway, P〈0.05）, small cell lung cancer and Endometrial cancer etc （human diseases pathway, P〈0.05）. Conclusions： miR-29a may be involved in the different signaling pathways via regulation of varies target genes and play an important role in the physiological and pathological processes. It is a valuable target for further investigation.