NaF·EDTA-K2抗凝对妊娠妇女血脂分析的影响

NaF·EDTA-K2 negatively bias blood lipids and lipoproteins measurement in pregnant women

目的：观察NaF · EDTA-K2抗凝对血脂分析的影响，探讨将妊娠妇女空腹 NaF · EDTA-K2抗凝血浆用于血脂分析的可行性。方法病例对照研究。收集2012年8至10月，广州市妇女儿童医疗中心珠江新城院区产科门诊及内分泌门诊的150例妊娠期妇女（年龄28.4±4.1岁，孕23-27周）的空腹NaF · EDTA-K2抗凝血浆和血清，采用配对t检验比较分析两组样本总胆固醇（CHO）、三酰甘油（TG）、高密度脂蛋白（HDL）、低密度脂蛋白（LDL）、载脂蛋白A1（apoA1）及载脂蛋白B（ apoB）的结果差异，采用直线回归分析两组样本血脂项目检测结果的相关性及血浆估计值标准误。结果 NaF· EDTA-K2抗凝血浆组各项目均值均略低于血清组，除TG外的5个项目结果存在统计学差异（LDL、apoA1、apoB P＜0.05；CHO、HDL P＜0.01）；经直线回归分析，两组标本各项目检测结果的相关系数分别为：CHO 0.968，TG 0.995，HDL 0.979，LDL 0.991，apoA10.692，apoB 0.846；血浆估计值的标准误分别为：CHO 0.281，TG 0.094，HDL 0.077，LDL 0.112，apoA10.230，apoB 0.111；相应修正系数为：CHO 1.08，HDL 1.08，LDL 1.08，ApoA11.14，ApoB 1.07。结论 NaF· EDTA-K2抗凝空腹血浆用于妊娠妇女血脂分析可造成微小负偏差，但检测结果与血清实测值有较好的相关性，经修正后可用于血脂水平预测。（中华检验医学杂志，2014，37：705-708）

Objective To explore the impact of NaF · EDTA-K2 on blood lipids measurement in pregnant women , determine whetherthe empty-stomach plasma for OGTT could be used as substitute for serumin blood lipids analyses.Methods Fastingplasma with NaF · EDTA-K2 and serumfrom 100 pregnant women werecollected,andconcentration of CHO,TG, HDL,LDL,apoA1 and apoB were tested with Hitachi 7 600 automatic biochemical analyzer.Data were analyzed by SPSS 19.0 software.Results The concentrations oflipids and lipoproteins with NaF · EDTA-K2 were slightly lower than those of serum.There were statistical significances between the means of CHO , HDL, LDL, apoA1 and apoBof two groups （ P〈0.05） except TG.Correlation coefficients of the six analyteswere CHO 0.968, TG 0.995, HDL 0.979, LDL 0.991, apoA1 0.692, apoB 0.846respectively.Standard Error of Estimate for plasma were： CHO 0.281, TG 0.094, HDL 0.077, LDL 0.112, apoA1 0.230, apoB 0.111 respectively.The modified coefficients were 1.08 for CHO, HDL and LDL, 1.14 for apoA1 and 1.07 for apoB.Conclusion NaF· EDTA-K2 negatively biased the blood lipidsmeasurement of CHO , HDL, LDL, apoA1 and apoB in pregnant women,yet lipids concentrations in plasma with NaF · EDTA-K2 were closely related to those in serum , which may be used to predict the bloodlipidslevel of pregnant women afterajusted.