三七皂苷R1对大鼠缺血心肌VEGF、bFGF的影响

Effects of Notoginsenoside R1 on VEGF and bFGF Expression in Ischemic Myocardium of Rats

目的:探讨三七皂苷R1对大鼠缺血心肌VEGF、bFGF的影响。方法:选择雄性Wistar大鼠39只,建立心肌梗死(AMI)模型,术后24h存活大鼠随机分为药物组(n=13)、对照组(n=13),另设假手术组(n=8)。药物组给予三七皂苷R1水溶液(2.5 mg·kg-1·d-1)腹腔注射、对照组及假手术组给予等体积生理盐水腹腔注射,用药4周。于实验终点处死大鼠,心肌组织取材,Ⅷ因子染色计数微血管数(MVC)及微血管密度(MVD),免疫组织化学法观察缺血心肌VEGF、bFGF蛋白的表达。结果:药物组及对照组MVC、MVD均高于假手术组,且药物组高于对照组(P<0.05);大鼠缺血心肌药物组及对照组VEGF、bFGF蛋白表达均高于假手术组(P<0.05),且药物组高于对照组(P<0.05)。结论:三七皂苷R1促进大鼠缺血心肌血管再生同时可上调缺血心肌VEGF、bFGF蛋白水平。

Objective： To study the effects ofnotoginsenoside R1 on the levels of vascular endothelial growth factor （VEGF） and basic fibroblast growth factor （bFGF） in ischemic myocardium of rats. Methods： The myocardial infarction （MI） model was established in thirty-nine male Wistar rats. 24h later, the surviving rats were randomly divided into drug group （n=13）, control group （n=13）, and sham-operated group （n=8）. Rats in the drug group were injected with notoginsenoside R1 （2.5 mg. kg.1. d-1） intrapefitoneally, while the control group and the sham-operated group received same volume of saline intraperitoneally. 4 weeks after treatment, the rats were euthanized for myocardial tissue samples. The miniature blood vessel （MVC） and microvascular density （MVD） were quantified according to F Ⅷ staining, and t, he expression of VEGF and bFGF were detected by immunohistochemistry. Results： MVC and MVD were increased in drug and control group compared with sham-operated group and the amount in drug group was higher than in the control group （P〈0.05）. The expression levels of VEGF and bFGF in ischemic myocardial drug group and control group were also higher than sham-operated group （P〈0.05） and the highest expression levels were observed in drug group （P〈0.05）. Conclusion： Notoginsenoside R1 can promote angiogenesis and increase the expression of VEGF and bFGF in ischemic myocardium of rats.