姜黄素对小鼠离体十二指肠平滑肌舒缩活动的影响

Effects of Curcumin on the Activity of Isolated Duodenal Smooth Muscle of Mice

目的:本研究采用小鼠离体十二指肠平滑肌观察姜黄素对胃肠道蠕动的影响,探讨其作用机制。方法:取小鼠离体十二指肠平滑肌条,放入37℃Krebs液浴槽中,通入95%氧气和5%二氧化碳混合气体,分组进行下列实验:对照组和分别加入10-40 M姜黄素组,测量记录十二指肠平滑肌的自主收缩变化;另取一组平滑肌条,分对照组、乙酰胆碱组、乙酰胆碱+姜黄素组、阿托品组、阿托品+姜黄素组,采用张力换能器连接多通道生理信号采集处理系统,测量比较十二指肠平滑肌舒缩的变化。结果:小鼠十二指肠平滑肌加入姜黄素孵育后,其自主收缩幅度有明显下降(P<0.01),而且降低的幅度与姜黄素剂量相关;给与乙酰胆碱引起十二指肠收缩后,再加姜黄素孵育,十二指肠平滑肌的收缩幅度明显的下降(P<0.01);给予阿托品引起小鼠平滑肌舒张后,再给予姜黄素孵育,平滑肌收缩幅度进一步降低。结论:姜黄素对小鼠离体十二指肠平滑肌具有直接舒张作用。

Objective： This study was to observe the effects of curcumin on the activity of isolated duodenal smooth muscle of mice. Methods： The duodenal smooth muscle strips of healthy mice were immediately removed and suspended in thermostatically control organ bath containing Krebs solution and to maintain continuously gassed with 95% 02 and 5% CO2. The experimental groups were divided into： Control and 10-40 M curcumin group individually. The spontaneous contraction of the strips was observed after incubated with different concentration of curcumin. Another duodenal smooth muscle strips were divided into： Control, acetylcholine （Ach）, Ach＋curcumin, atropine, and atropin＋curcumin groups. The changes of duodenal contractionor relaxation were observed and compared y tonotransducer connected with signal acquization system. Results： After the duodenal smooth muscle strips were incubated with curcumin in 20 μmol/L-40 μmol/L, the mean contractile amplitude of the duodenal smooth muscle strips was significantly reduced （P〈0.05）in a dose-dependent fashion. After adding Ach to induce duodenal contraction, the mean contractile amplitude of the duodenal muscle was obviously reduced by curcumin （P〈0.05 or P〈0.01）. After adding atropine to induce duodenal relaxation, the mean contractile amplitude of the duodenal smooth muscle was also reduced （P〈0.05）. Conclusion： Curcumin directly induce relaxation on isolated duodenal smooth muscle of mice.