新型可降解PEI衍生物的表征及其在Brl-3A细胞中的毒性和转染研究

Characterizations of Novel Degradable Polyethyleneimine Derivatives and Transfection and Cytotoxicity Studies on Brl-3A

目的:对新型可降解高分子进行表征,研究其在Brl-3A细胞中的毒性和转染效率,以及连接剂比例对以上方面的影响。方法:通过化学方法合成不同比例PEI-Tr高分子,考察其包裹质粒DNA形成纳米颗粒的粒径和电位,以CCK-8方法考察Brl-3A细胞中的细胞毒性,以荧光素酶质粒为报告基因考察Brl-3A细胞中的转染效率。结果:PEI-Tr材料能形成200 nm以下带20 mV左右正电荷的纳米颗粒,具有较好的细胞内吞能力和溶液稳定性,细胞毒性实验证明,随着浓度增加PEI-Tr材料显示了远低于PEI-25kDa的细胞毒性,细胞转染实验表明其拥有高效输送质粒的能力。结论:PEI-Tr是一种高效低毒的可降解聚阳离子载体,在基因输送领域有很大的潜力;连接剂的比例在聚阳离子功能中起到重要作用。

Objective： To characterize a novel biodegradable polymer, investigate its cell cytotoxicity and gene transfection efficiency, and the influence of linker＇s ratio. Methods： PEI-Tr series were synthesized, and the particle size and Zeta potential of polymer-DNA nanoparticles were estimated, then, cell cytotoxicity and transfection efficiency were tested. Results： PEI-Tr could form nanoparticles under 200 nm, and its Zeta potential was about 20 mV, which indicated that it had a good cell endocytosis capability and stability. The Brl-3A cytotoxicity and transfection results suggested that PEI-Tr had a high plasmid transfection ability within low cell toxicity. Conclusion： PEI-Tr is a promising, efficient and low-toxic gene carrier, and the linker ratio is an important factor in carrier design.