腺病毒携带IL-12增强抗原致敏树突细胞在肝癌基因治疗中的研究

Adenovirus Mediated Interleukin-12 Enhances the Gene Therapy of Hepatocarcinoma by Tumor Antigen Plused Dendritic Cells

目的:近年来通过应用白介素-12治疗肿瘤取得良好效果,因此对国内外应用腺病毒携带IL-12增强抗原致敏树突细胞在肝癌基因治疗中的研究进展进行总结,以探索更为可行治疗方法。方法:运用Pubmed、Elsevier Sciencedirect、CNKI及万方全文数据库检索系统,以腺病毒,IL-12,肝癌,树突细胞为关键词,检索2008-01至2012-11月发表的文献。纳入标准:1)IL-12的生物学特性,在抗肿瘤过程中的免疫作用,2)应用腺病毒携带IL-12对抗肝癌治疗研究,3)肿瘤抗原致敏树突细胞对肿瘤的影响。根据纳入标准分析文献26篇。结果:通过腺病毒携带IL-12可以增强肿瘤抗原致敏树突细胞的免疫应答。并通过诱导肿瘤细胞的凋亡,减少新生血管的生成而对肿瘤产生直接抑制,有效抑制肝癌的生长和转移。结论:本文通过对IL-12的生物学特征、抗肿瘤通路、作用机制及在腺病毒介导下肿瘤抗原致敏树突细胞研究进展的概述,为腺病毒携带IL-12作为肝癌的基因治疗进一步提供理论依据和探索,期待在将来应用IL-12为基础的基因治疗一定会为包括肝癌在内的肿瘤治疗提供新的途径。

Objective： Recently, wonderful effect has been achieved on treating tumor by using IL-12. So we summarize the advances of adnovirus mediated interleukin-12 enhances the gene therapy of hepatocarcinoma by tumor plused dendritic cells at home and abroad on the purpose of finding a feasible way for treating tumors. Methods： Using adenovirus IL-12,hepatocarcinoma and dendritic cells as key words, the articles published from the database such as Pubmed. Elsevier Sciencedirect, CNKI and Wanfang, particularly from January 2008 to November 2011, were searched. Inclusion criteria： 1）the biologic character of IL-12 and the immune effect on the progress of anti-tumor, 2）the reserch of using adenovirus mediated IL-12 to therapy tumor, 3）The influnce of tumor antigen pulsed dendritic cell on tumor. Twenty-six articles were selected into analysis according to inclusion criteria. Results： The immune response can be improved by using adenovirus mediated IL-12 enhances tumor antigen pulsed dendritic cells. Tumors were depressed directly by inducing the tumor＇s apoptosis and reducing the angiogenesis, which can depress the growth and metastasis of the hepatocarcinoma effectively. Conclusions： This article conclude the biologic character, the pathway and mechanism of anti-tumor of IL-12 and the advance of the stdudy of adenovirus meditated tumor antigen pulsed dendritic cells, which also can provide the theoretical evidence for further exploration of the hepatocarcinoma gene therapy by adenovirus mediated IL-12. And we hope to provide a novel approach for the tumor treating including hepatocarcinoma, on the basis ofgene cure of IL-12 in the future.