成纤维细胞生长因子2、成纤维细胞生长因子受体4蛋白在人甲状腺乳头状癌中的表达及意义

Expression of fibroblast growth factor-2 and fibroblast growth factor receptor-4 in human papillary thyroid carcinoma and their significances

目的探讨甲状腺乳头状癌(PTC)组织中成纤维细胞生长因子2(FGF-2)和成纤维细胞生长因子受体4(FGFR-4)的表达及其是否存在相关性。方法收集89例甲状腺乳头状癌及30例癌旁正常甲状腺组织标本,采用免疫组织化学和免疫印迹法(Western blotting)检测FGF-2和FGFR-4蛋白在标本中的表达,并进行统计学分析。结果免疫组织化学方法结果显示,与癌旁正常组织相比,FGF-2及FGFR-4在人类甲状腺乳头状癌组织中均高表达(P<0.01,P<0.01),两者差异有统计学意义;FGF-2和FGFR-4在甲状腺乳头状癌中的表达与淋巴结转移(χ2=14.798,P<0.01;χ2=7.27,P<0.01)和分化程度(χ2=13.824,P<0.01;χ2=16.921,P<0.01)相关,而与性别、年龄、肿瘤大小无关(P>0.05);通过Western blotting技术分析,FGF-2和FGFR-4癌组织中的表达明显高于正常组织,随着癌组织分化程度的降低,表达明显上调(P<0.05),其结果和免疫组织化学染色的检测结果一致;并且两者在甲状腺乳头状癌中的表达呈正相关(rs=0.434,P<0.01)。结论 FGF-2和FGFR-4与甲状腺乳头状癌的发生、侵袭和转移有关,两者具有正协同作用,联合检测对判断甲状腺乳头状癌的恶性程度及生物学行为是一项有意义的综合性指标。

Objective To investigate the expression of fibroblast growth factor-2 （FGF-2） and fibroblast growth factor receptor-4 （ FGFR-4 ） in the papillary thyroid carcinomas （ PTC ） and clinical significance . Methods Immunohistochemistry and Western blotting for the expression of FGF-2 and FGFR-4 were performed in 89 cases of PTC and 30 cases of normal thyroid tissues （ NTT） adjacent to the tumors .Results Immunohistochemistry results showed that , FGF-2 and FGFR-4 expressions were high in thyroid carcinoma （P〈0.01,P〈0.01） in contrast to that in the normal thyroid tissues, and the difference was statistically significant;There was a positive linear correlation between expressions of FGF-2 and FGFR-4 and lymph node metastasis （χ2 =14.798,P〈0.01;χ2 =7.27,P〈0.01）and differentiation degree （χ2=13.824,P〈0.01;χ2 =16.921, P〈0.01） in papillary thyroid carcinoma ,while there was no difference in gender ,age and tumor size（P〉0.05）.Analyzed by Western blotting technique ,FGF-2 and FGFR-4 expressions in thyroid carcinoma were significantly higher than that in normal tissue ,with decrease of cancer degree of tissue differentiation and significantly up regulated expression （P〈0.05）.Expressions of FGF-2 and FGFR-4 were in a positive linear correlation in the disease （rs=0.434,P〈0.01）.Conclusion The expressions of FGF-2 and FGFR-4 are correlated with papillary thyroid cancer and they participated in the process of invasion and metastasis , both of which have a positive synergistic effect .The degree of malignancy and biological behavior are meaningful and comprehensive indicators ,which provide a theoretical basis for the subsequent experimental studies of cellular and molecular biology .