摘要
The grass carp(Ctenopharyngodon idella) is one of the most important cultivated fish species in China.Mounting evidences suggests that microRNAs(miRNAs) may be key regulators of skeletal muscle among the grass carp,but the knowledge of the identity of myogenic miRNAs and role of miRNAs during skeletal muscle anabolic state remains limited.In the present study,we choose 8 miRNAs previously reported to act as muscle growth-related miRNAs for fasting-refeeding research.We investigated postprandial changes in the expression of 8 miRNAs following a single satiating meal in grass carp juveniles who had been fasting for one week and found that 7 miRNAs were sharply up-regulated within 1 or 3 h after refeeding,suggesting that they may be promising candidate miRNAs involved in a fast-response signaling system that regulates fish skeletal muscle growth.
The grass carp （Ctenopharyngodon idella） is one of the most important cultivated fish species in China. Mounting evidences suggests that microRNAs （miRNAs） may be key regulators of skeletal muscle among the grass carp, but the knowledge of the identity of myogenic miRNAs and role of miRNAs during skeletal muscle anabolic state remains limited. In the present study, we choose 8 miRNAs previously reported to act as muscle growth-related miRNAs for fasting-refeeding research. We investigated postprandial changes in the expression of 8 miRNAs following a single satiating meal in grass carp juveniles who had been fasting for one week and found that 7 miRNAs were sharply up-regulated within 1 or 3 h after refeeding, suggesting that they may be promising candidate miRNAs involved in a fast-response signaling system that regulates fish skeletal muscle growth.
基金
Foundation items： This study was supported by the National Natural Science Foundation of China （31230076
31340054）, the Natural Science Foundation of Hunan province （14JJ2135）and the State Key Laboratory of Freshwater Ecology and Biotechnology （2012FB01 ）
