成都地区女性献血者HLA抗体分布

Prevalence of HLA antibodies in female blood donors in Chengdu,China

目的筛查女性献血者HLA-Ⅰ/Ⅱ抗体,了解成都地区女性献血者HLA抗体阳性率。方法 2013年2月～2014年1月随机选取女性献血者298名献血者标本,采用HLA抗体筛查试剂盒,检测HLAⅠ/Ⅱ抗体。结果筛查出阳性献血者74名（24.83%）,其中HLA-Ⅰ单阳58人（19.46%）,HLA-Ⅱ单阳7人（2.35%）,双阳9人（3.02%）。电话回访65名献血者,均有妊娠史,怀孕1次7人,2次21人,3次及以上36人,其中3人有输血史。结论成都地区女性献血者HLA阳性率与部分地区报道一致,HLA抗体阳性构成比随怀孕次数增加而呈上升趋势。筛查有妊娠史女性单采血小板献血者HLA抗体十分必要。

Objective To assess the prevalence of HLA- Ⅰ/Ⅱ antibodies in female blood donors. Methods Blood samples from 298 women blood donors were randomly collected from February 2013 to January 2014, using HLA antibody screening kit to detect the HLA antibodies. Rtsdts A total of 298 donors were tested. There were 74 （24.83%） HLA antbody positive blood donors ,of which 58 （ 19.46% ） were anti HLA class Ⅰ positive donors ,7 （ 2.35% ） were anti HLA class Ⅱ donors,and 9（3.02% ）were anti HLA classⅠ and Ⅱ positive donors. Telephone interviews were conducted with 65 do- nors,of which all had history of pregnancy. The number of donors who have been pregnant once, twice, three times and more were 7,21 and 36,respectively. Among those 65 donors interviewed, three had history of blood transfusion. Conclusion The HLA antibodies positive rate of female donors in Chengdu is consistent with those reported in some areas. HLA antibodies positive ratio elevates with the increasing number of pregnancy. It is necessary to screen HLA antibodies in the female apheresis platelet donors who had history of pregnancy.